Optical birefringence of liquid crystals for label-free optical biosensing diagnosis
- Authors
- Nguyen, Tan Tai; Han, Gyeo-Re; Jang, Chang-Hyun; Ju, Heongkyu
- Issue Date
- Aug-2015
- Publisher
- DOVE MEDICAL PRESS LTD
- Keywords
- biological sensors; LC orientation; protein sensing; tag-free diagnosis
- Citation
- INTERNATIONAL JOURNAL OF NANOMEDICINE, v.10, pp.25 - 32
- Journal Title
- INTERNATIONAL JOURNAL OF NANOMEDICINE
- Volume
- 10
- Start Page
- 25
- End Page
- 32
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/11982
- DOI
- 10.2147/IJN.S88286
- ISSN
- 1178-2013
- Abstract
- Purpose: We present a polarization-sensitive optical detection platform for label-free quantitative optical biosensing diagnosis using liquid crystals (LCs). This is capable of determining quantitatively the optical birefringence of optical cells containing LCs, whose orientation depends on the immobilized biomolecules. Patients and methods: This technique uses a polarization-dependent double-port detection without any polarizer at a single wavelength and removes the need of aligning optical cells of LCs in the azimuthal direction, with respect to the light path through the optical cell. Thus, this technique enables a stand-alone detection in a relatively compact format without an additional optical instrument, such as a retardation compensator, a Michael-Levy chart, and a spectrophotometer, in order to determine the optical birefringence quantitatively. Results: We demonstrate that bovine serum albumin immobilized on the gold surface of the cell hybrid interfaces that support both homeotropic and planar anchoring of LCs causes optical phase retardation change which can be determined quantitatively. We also provide estimation of the zenithal orientation of LCs near the gold surface of the hybrid interfaces, based on the phase retardation determined. The estimated limit of bovine serum albumin detection is approximately 2.1 mu M. Conclusion: This optical technique with LCs can serve an optical platform for label-free quantitative diagnosis of proteins in a real time manner.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 나노물리학과 > 1. Journal Articles
- 바이오나노대학 > 나노화학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/11982)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.