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A capacitive biosensor based on an interdigitated electrode with nanoislands
- Issue Date
- 24-Sep-2014
- Publisher
- ELSEVIER
- Keywords
- Interdigitated electrode; Nanoisland; Capacitive biosensor; Hepatitis B virus; Surface antigen; Immunosensor
- Citation
- ANALYTICA CHIMICA ACTA, v.844, pp.27 - 34
- Journal Title
- ANALYTICA CHIMICA ACTA
- Volume
- 844
- Start Page
- 27
- End Page
- 34
- ISSN
- 0003-2670
- Abstract
- A capacitive biosensor based on an interdigitated electrode (IDE) with nanoislands was developed for label-free detection of antigen-antibody interactions. To enable sensitive capacitive detection of protein adsorption, the nanoislands were fabricated between finger electrodes of the IDE. The effect of the nanoislands on the sensitive capacitive measurement was estimated using horseradish peroxidase (HRP) as a model protein. Additionally, a parylene-A film was coated on the IDE with nanoislands to improve the efficiency of protein immobilization. By using HRP and hepatitis B virus surface antigen (HBsAg) as model analytes, the effect of the parylene-A film on the capacitive detection of protein adsorption was demonstrated. (C) 2014 Elsevier B.V. All rights reserved.
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