Clinical Availability of Component-Resolved Diagnosis Using Microarray Technology in Atopic Dermatitis
- Authors
- Choi, Joon-Seok; Roh, Joo-Young; Lee, Jong-Rok
- Issue Date
- Aug-2014
- Publisher
- KOREAN DERMATOLOGICAL ASSOC
- Keywords
- Atopic dermatitis; Microarray-based specific IgE detection assay
- Citation
- ANNALS OF DERMATOLOGY, v.26, no.4, pp.437 - 446
- Journal Title
- ANNALS OF DERMATOLOGY
- Volume
- 26
- Number
- 4
- Start Page
- 437
- End Page
- 446
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/12458
- DOI
- 10.5021/ad.2014.26.4.437
- ISSN
- 1013-9087
- Abstract
- Background: Various allergens play a role in the elicitation or exacerbation of eczematous skin lesions in atopic dermatitis (AD), and much research effort has been focused on improving diagnostic tests to identify causative allergens. Objective: The purpose of this study was to evaluate the diagnostic effectiveness of a newly introduced microarray-based specific immunoglobulin E detection assay, Immuno-CAP ISAC, for use in AD patients. Methods: The serum samples of 25 AD patients were tested by using ISAC and a multiple allergen simultaneous test-enzyme immunoassay (MAST-EIA). In addition, 10 of the 25 patients underwent skin prick testing (SPT). The positive reaction rates to allergens in each test and the agreements, sensitivities, and specificities of ISAC and MAST-EIA were evaluated versus the SPT results. Results: For ISAC versus SPT, the overall results were as follows: sensitivity, 90.0%; specificity, 98.2%; positive predictive value (PPV), 90.0%; and negative predictive value (NPV), 98.2%. The total agreement and K value for ISAC versus SPT were 96.9% and 0.882, respectively. For MAST-EIA versus SPT, the sensitivity was 80.0%, specificity 92.7%, PPV 66.7%, and NPV 96.2%. The total agreement and K value for MAST-EIA versus SPT were 90.8% and 0.672, respectively. The overall agreement between the ISAC and MAST-EIA results was 88%. Conclusion: The ISAC results in AD correlated well with the SPT results, and compared favorably to the MAST-EIA results. This study demonstrates the potential of ISAC as a convenient allergic diagnostic method in AD patients.
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