Effects of agmatine and resveratrol on RGC-5 cell behavior under light stimulation

Abstract

A light radiation causes dysfunction and death of retinal cells and leads to degeneration. Present study, investigated the light-induced cell dysfunction, and their activity. Further, the effects of agmatine and resveratrol on light-induced damage and these underlying photo-oxidative and protective mechanisms were monitored by real-time bio-impedance system. After light exposure retinal ganglion cells underwent death in a time dependent manner. During light exposure the cells elevate free radicals and Ca2+, followed by nitric oxide (NO) and tumor necrosis factor-alpha (TNF-alpha), which can be facilitated to cell demise. The results revealed that these drugs can control the elevation of free radical, calcium gating, NO level, and increased TNF-alpha, which could diminish cell photo-damage. In summary, resveratrol helps more to rescue damaged cells compared to agmatine. The proposed system suggested mechanism could meet to identify the photo-toxic effects in retinal cells, and provides high throughput screening for early stages photo-damage. (C) 2014 Elsevier B.V. All rights reserved.