3'-Deoxy-3'-[F-18]Fluorothymidine Positron Emission Tomography Imaging of Thymidine Kinase 1 Activity After 5-Fluorouracil Treatment in a Mouse Tumor Model
- Authors
- Hong, Il Ki; Kim, Seog Young; Chung, Jin Hwa; Lee, Seung Jin; Oh, Seung Jun; Lee, Sang Ju; Oh, Jungsu; Ryu, Jin-Sook; Kim, Tae Won; Kim, Deog Yoon; Moon, Dae Hyuk
- Issue Date
- Feb-2014
- Publisher
- INT INST ANTICANCER RESEARCH
- Keywords
- 3' -deoxy-3' -[18F]fluorothymidine; positron emission tomography; thymidine kinase; 5-fluorouracil; thymidylate synthase
- Citation
- ANTICANCER RESEARCH, v.34, no.2, pp.759 - 766
- Journal Title
- ANTICANCER RESEARCH
- Volume
- 34
- Number
- 2
- Start Page
- 759
- End Page
- 766
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/12875
- ISSN
- 0250-7005
- Abstract
- Aim: We aimed to investigate whether 3'-deoxy-3'-[F-18]fluorothymidine ([F-18]FLT) positron emission tomography (PET) can estimate thymidine kinase 1 (TK1) activity after thymidylate synthase (TS) inhibition by 5-fluorouracil (5-FU) in a mouse tumor model. Materials and Methods: Nude mice with HT29 tumors were injected with phosphate-buffered saline or 5-FU (16.7 or 50 mg/kg). Twenty-four hours later, 2-hour dynamic images were acquired after injection of [F-18]FLT. In another group of mice with HT29 cells, static PET images were obtained 110 min after [F-18]FLT injection. Results: Kinetic parameters related to [F-18]FLT retention increased significantly, whereas the de-phosphorylation of [F-18]FLT monophosphate decreased significantly. The standardized uptake value (SUVmean) of HT29 tumors correlated significantly with the net influx constant and the distribution volume for phosphorylated [F-18]FLT. There was a significant correlation between the tumor SUVmean and TK1 activity. Conclusion: SUVmean at 110-120 mm after [F-18]FLT, can quantitatively evaluate kinetic parameters and TK1 activity after TS inhibition.
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