Double-layer PDMS microdevice for parallel continuous-flow PCR employing a single heater for temperature control and plastic syringe for sample actuation
DC Field | Value | Language |
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dc.contributor.author | Trinh, K.T.L. | - |
dc.contributor.author | Ha, M.L. | - |
dc.contributor.author | Wu, W. | - |
dc.contributor.author | Lee, N.Y. | - |
dc.date.available | 2020-02-28T18:47:11Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 0000-0000 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/13111 | - |
dc.description.abstract | We report on a 2-temperature on-chip continuous-flow PCRs in a 3D fluidic configuration employing a single heater and a portable plastic syringe, and demonstrate two parallel reactions utilizing a single microdevice. To investigate the feasibility of employing a single heater as well as to assess the reliability of the sample injection performance initiated by the plastic syringes, on-chip continuous-flow PCRs were performed using plasmid DNA as templates inside 2 meter-long spiral microchannels constructed in a double-layer arrangement. Target amplicons were successfully amplified simultaneously in parallel reactions inside the microdevice in approximately 27 min with high reproducibility. © 14CBMS. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Chemical and Biological Microsystems Society | - |
dc.relation.isPartOf | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 | - |
dc.subject | Microchannels | - |
dc.subject | Parallel flow | - |
dc.subject | Syringes | - |
dc.subject | Continuous flows | - |
dc.subject | Double layers | - |
dc.subject | High reproducibility | - |
dc.subject | Long spirals | - |
dc.subject | Micro-device | - |
dc.subject | Parallel reactions | - |
dc.subject | Plastic syringes | - |
dc.subject | Sample injection | - |
dc.subject | Polymerase chain reaction | - |
dc.title | Double-layer PDMS microdevice for parallel continuous-flow PCR employing a single heater for temperature control and plastic syringe for sample actuation | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.bibliographicCitation | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014, pp.1024 - 1026 | - |
dc.identifier.scopusid | 2-s2.0-84941662708 | - |
dc.citation.endPage | 1026 | - |
dc.citation.startPage | 1024 | - |
dc.citation.title | 18th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2014 | - |
dc.contributor.affiliatedAuthor | Trinh, K.T.L. | - |
dc.contributor.affiliatedAuthor | Ha, M.L. | - |
dc.contributor.affiliatedAuthor | Wu, W. | - |
dc.contributor.affiliatedAuthor | Lee, N.Y. | - |
dc.type.docType | Conference Paper | - |
dc.subject.keywordAuthor | Continuous-flow polymerase chain reaction | - |
dc.subject.keywordAuthor | Plastic syringe | - |
dc.subject.keywordAuthor | Sample actuation | - |
dc.subject.keywordAuthor | Three-Dimensional microdevice | - |
dc.subject.keywordPlus | Microchannels | - |
dc.subject.keywordPlus | Parallel flow | - |
dc.subject.keywordPlus | Syringes | - |
dc.subject.keywordPlus | Continuous flows | - |
dc.subject.keywordPlus | Double layers | - |
dc.subject.keywordPlus | High reproducibility | - |
dc.subject.keywordPlus | Long spirals | - |
dc.subject.keywordPlus | Micro-device | - |
dc.subject.keywordPlus | Parallel reactions | - |
dc.subject.keywordPlus | Plastic syringes | - |
dc.subject.keywordPlus | Sample injection | - |
dc.subject.keywordPlus | Polymerase chain reaction | - |
dc.description.journalRegisteredClass | scopus | - |
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