Double-layer PDMS microdevice for parallel continuous-flow PCR employing a single heater for temperature control and plastic syringe for sample actuation

Abstract

We report on a 2-temperature on-chip continuous-flow PCRs in a 3D fluidic configuration employing a single heater and a portable plastic syringe, and demonstrate two parallel reactions utilizing a single microdevice. To investigate the feasibility of employing a single heater as well as to assess the reliability of the sample injection performance initiated by the plastic syringes, on-chip continuous-flow PCRs were performed using plasmid DNA as templates inside 2 meter-long spiral microchannels constructed in a double-layer arrangement. Target amplicons were successfully amplified simultaneously in parallel reactions inside the microdevice in approximately 27 min with high reproducibility. © 14CBMS.