마우스 대식세포를 이용한 어성초(魚腥草) 물추출물의 항염효능연구Anti-inflammatory Effect of Houttuyniae Herba Water Extract on LPS-induced RAW 264.7 Mouse Macrophages
- Other Titles
- Anti-inflammatory Effect of Houttuyniae Herba Water Extract on LPS-induced RAW 264.7 Mouse Macrophages
- Authors
- 황인승; 김영진; 박윤수; 김현주; 김도훈; 박완수
- Issue Date
- 2014
- Publisher
- 대한본초학회
- Keywords
- Houttuyniae Herba; Macrophage; Inflammation; Cytokine; Nitric oxide; Calcium
- Citation
- 대한본초학회지, v.29, no.4, pp.83 - 89
- Journal Title
- 대한본초학회지
- Volume
- 29
- Number
- 4
- Start Page
- 83
- End Page
- 89
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/13566
- ISSN
- 1229-1765
- Abstract
- Objectives : The purpose of this study was to investigate effects of Houttuyniae Herba water extract (HC) on calcium release and production of various inflammatory mediators such as nitric oxide (NO), interferon-inducible protein (IP)-10, platelet derived growth factor (PDGF)-BB, keratinocyte-derived chemokine (KC), vascular endothelial growth factor (VEGF), interleukin (IL)-4, and IL-5 in lipopolysaccharide (LPS)-induced RAW 264.7 mouse macrophages.
Methods : NO production was measured by Griess reagent assay. Intracellular calcium level was measured with Fluo-4 assay. Levels of cytokines were measured by High-throughput multiplex bead array cytokine assay based on xMAP (multi-analyte profiling beads) technology.
Results : HC significantly decreased NO production for 24 hrs incubation at the concentrations of 10, 25, 50, 100, and 200 ㎍/mL in LPS-induced RAW 264.7 (P < 0.05). HC significantly decreased production of IP-10, KC, VEGF, and PDGF-BB for 24 hrs incubation at the concentrations of 50, 100, and 200 ㎍/mL in LPS-induced RAW 264.7 (P < 0.05). HC also significantly decreased intracellular calcium release for 24 hrs incubation at the concentrations of 25, 50, 100, and 200 ㎍/mL in LPS-induced RAW 264.7 (P < 0.05). But HC did not show any significant effect on production of IL-4 and IL-5 in LPS-induced RAW 264.7.
Conclusions : The results suggested that HC has anti-inflammatory property related with its inhibition on the production of NO, IP-10, KC, VEGF, and PDGF-BB in LPS-induced macrophages via calcium pathway.
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