Isolation and Characterization of a Bacterial Isolate for Polyhydroxyalkanoates Production

Abstract

Microorganism accumulating polyhydroxyalkanoates (PHA) in its cytosol was isolated from water obtained from InCheonarea. This strain was determined to be Bacillus species that was examined by 16S rDNA sequences and phylogenetic treeanalysis. The bacteria accumulated up to 20.6% of PHA within 48 hours at 37oC in the presence of 2.0% glucose substrate underaerobic condition. The present work also investigates the influence of substrate on PHA production to find a clue trigger. ThePHA accumulation increased to 23.5 and 23.3% containing 2.0% salicylic acid and lactose, respectively. In contrast both glucosamineand xylose have negative effect on PHA production for this bacterial strain. To the best of our knowledge, this reportfirstly demonstrates the effects of various sugars on PHA production.