梔子가 대식세포의 Cytokine에 미치는 영향
DC Field | Value | Language |
---|---|---|
dc.contributor.author | 차지혜 | - |
dc.contributor.author | 임은미 | - |
dc.date.available | 2020-02-28T21:42:47Z | - |
dc.date.created | 2020-02-12 | - |
dc.date.issued | 2014 | - |
dc.identifier.issn | 1229-4292 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/13824 | - |
dc.description.abstract | Objectives: The purpose of this study was to investigate the effects of GardeniaeFructus Water Extract (GF) on the production of inflammatory mediators in RAW264.7 cell treated with lipopolysaccharide (LPS). Methods: Gradeniae Fructus was extracted with distilled water (2,000 ml) for 2hours. In order to evaluate cytotoxicity of GF, 3 - (4,5-dimethylthiazol-2-yl) - 2,5- diphenyltetrazolium bromide (MTT) assay was performed. To investigate antiinflammatoryeffects, the concentration of nitric oxide (NO) was measured with Noassay, calcium (Ca) was measured with Fluo-4 Ca assay, and cytokine was measuredby Bio-Plex cytokine assay in RAW 264.7 cell. And when p-value is below 0.05, itis judged to have the significant difference statistically. Results:1. GF did not show any cytotoxicity. 2. GF suppressed the production of NO and Ca at the concentration of 25, 50,100 and 200 μg/ml. 3. GF suppressed the production of interleukin (IL)-1β, IL-10, IL-12p40, macrophage-colony stimulating factor (M-CSF), macrophage inflammatory protein (MIP)-1β andkeratinocyte chemoattractant(KC) at the concentration of 25, 50, 100 and 200 μg/ml. 4. GF suppressed the production of vascular endothelial growth factor (VEGF),granulocyte-colony stimulating factor (G-CSF) and monocyte cheomattractant protein(MCP)-1 at the concentration of 25, 50 and 100 μg/ml. 5. GF suppressed the production of granulocyte macrophage-colony stimulatingfactor (GM-CSF) and regulated on activation, normal T cell expressed and secreted(RANTES) at the concentration of 25 and 50 μg/ml. 6. GF suppressed the production of MIP-2 at the concentration of 50 and 100μg/ml, and tumor necrosis factor (TNF)-α at the concentration of 50 and 200 μg/ml. Conclusions: These results suggest that GF has anti-inflammatory effect andimmuno-modulating activity. | - |
dc.language | 한국어 | - |
dc.language.iso | ko | - |
dc.publisher | 대한한방부인과학회 | - |
dc.relation.isPartOf | 대한한방부인과학회지 | - |
dc.title | 梔子가 대식세포의 Cytokine에 미치는 영향 | - |
dc.title.alternative | Effects of Gardeniae Fructus on Cytokines in Mouse Macrophage | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 2 | - |
dc.identifier.doi | 10.15204/jkobgy2014.27.1.001 | - |
dc.identifier.bibliographicCitation | 대한한방부인과학회지, v.27, no.1, pp.1 - 16 | - |
dc.identifier.kciid | ART001851185 | - |
dc.citation.endPage | 16 | - |
dc.citation.startPage | 1 | - |
dc.citation.title | 대한한방부인과학회지 | - |
dc.citation.volume | 27 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | 차지혜 | - |
dc.contributor.affiliatedAuthor | 임은미 | - |
dc.subject.keywordAuthor | Gardeniae Fructus | - |
dc.subject.keywordAuthor | Macrophage | - |
dc.subject.keywordAuthor | Anti-inflammation | - |
dc.subject.keywordAuthor | NO | - |
dc.subject.keywordAuthor | Cytokine | - |
dc.description.journalRegisteredClass | kci | - |
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