梔子가 대식세포의 Cytokine에 미치는 영향Effects of Gardeniae Fructus on Cytokines in Mouse Macrophage
- Other Titles
- Effects of Gardeniae Fructus on Cytokines in Mouse Macrophage
- Authors
- 차지혜; 임은미
- Issue Date
- 2014
- Publisher
- 대한한방부인과학회
- Keywords
- Gardeniae Fructus; Macrophage; Anti-inflammation; NO; Cytokine
- Citation
- 대한한방부인과학회지, v.27, no.1, pp.1 - 16
- Journal Title
- 대한한방부인과학회지
- Volume
- 27
- Number
- 1
- Start Page
- 1
- End Page
- 16
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/13824
- DOI
- 10.15204/jkobgy2014.27.1.001
- ISSN
- 1229-4292
- Abstract
- Objectives: The purpose of this study was to investigate the effects of GardeniaeFructus Water Extract (GF) on the production of inflammatory mediators in RAW264.7 cell treated with lipopolysaccharide (LPS).
Methods: Gradeniae Fructus was extracted with distilled water (2,000 ml) for 2hours. In order to evaluate cytotoxicity of GF, 3 - (4,5-dimethylthiazol-2-yl) - 2,5- diphenyltetrazolium bromide (MTT) assay was performed. To investigate antiinflammatoryeffects, the concentration of nitric oxide (NO) was measured with Noassay, calcium (Ca) was measured with Fluo-4 Ca assay, and cytokine was measuredby Bio-Plex cytokine assay in RAW 264.7 cell. And when p-value is below 0.05, itis judged to have the significant difference statistically.
Results:1. GF did not show any cytotoxicity.
2. GF suppressed the production of NO and Ca at the concentration of 25, 50,100 and 200 μg/ml.
3. GF suppressed the production of interleukin (IL)-1β, IL-10, IL-12p40, macrophage-colony stimulating factor (M-CSF), macrophage inflammatory protein (MIP)-1β andkeratinocyte chemoattractant(KC) at the concentration of 25, 50, 100 and 200 μg/ml.
4. GF suppressed the production of vascular endothelial growth factor (VEGF),granulocyte-colony stimulating factor (G-CSF) and monocyte cheomattractant protein(MCP)-1 at the concentration of 25, 50 and 100 μg/ml.
5. GF suppressed the production of granulocyte macrophage-colony stimulatingfactor (GM-CSF) and regulated on activation, normal T cell expressed and secreted(RANTES) at the concentration of 25 and 50 μg/ml.
6. GF suppressed the production of MIP-2 at the concentration of 50 and 100μg/ml, and tumor necrosis factor (TNF)-α at the concentration of 50 and 200 μg/ml.
Conclusions: These results suggest that GF has anti-inflammatory effect andimmuno-modulating activity.
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