NF-κB Inhibitor Suppresses Hypoxia-induced Apoptosis of Mouse Pancreatic β-cell Line MIN6
- Authors
- 고현숙; 김재영
- Issue Date
- Mar-2014
- Publisher
- 대한의생명과학회
- Keywords
- NF-kappaB; Cell hypoxia; Insulin-secreting cells; Apoptosis; Chemokine CXCL10
- Citation
- 대한의생명과학회지, v.20, no.1, pp.14 - 24
- Journal Title
- 대한의생명과학회지
- Volume
- 20
- Number
- 1
- Start Page
- 14
- End Page
- 24
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/13963
- Abstract
- attempted to determine the potential usefulness of NF-κB inhibitor for suppression of hypoxia-induced β-cellapoptosis as well as the relationship between IP-10 induction and β-cell apoptosis in hypoxia. To accomplish this, wecultured the mouse pancreatic β-cell line MIN6 in hypoxia (1% O2). Among several examined chemokines, only IP-10mRNA expression was induced under hypoxia, and this induced IP-10 expression was due to NF-κB activity. Since aprevious study suggested that IP-10 mediates β-cell apoptosis, we measured hypoxia-induced IP-10 protein and examinedthe effect of anti-IP-10 neutralizing Ab on hypoxia-induced β-cell apoptosis. However, IP-10 protein was not detected,and anti-IP-10 neutralizing Ab did not rescue hypoxia-induced MIN6 apoptosis, indicating that there is no relationshipbetween hypoxia-induced IP-10 mRNA expression and hypoxia-induced β-cell apoptosis. Since it was still not clear ifNF-κB functions as an apoptotic or anti-apoptotic mediator in hypoxia-induced β-cell apoptosis, we examined possibleinvolvement of NF-κB in hypoxia-induced β-cell apoptosis. Treatment with 1 μM NF-κB inhibitor suppressed hypoxiainducedapoptosis by more than 50%, while 10 μM AP-1 or 4 μM NF-AT inhibitor did not, indicating involvement ofNF-κB in hypoxia-induced β-cell apoptosis. Overall, these results suggest that IP-10 is not involved in hypoxia-inducedβ-cell apoptosis, and that NF-κB inhibitor can be useful for ameliorating hypoxia-induced β-cell apoptosis.
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