Effects of human epidermal growth factor gene-transfected mesenchymal stem cells on fibroblast migration and proliferation
DC Field | Value | Language |
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dc.contributor.author | You, D. H. | - |
dc.contributor.author | Nam, M. J. | - |
dc.date.available | 2020-02-28T23:43:41Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2013-08 | - |
dc.identifier.issn | 0960-7722 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14409 | - |
dc.description.abstract | ObjectivesWe were interested in determining whether epidermal growth factor gene-transfected mesenchymal stem cells (EGF-MSC) would accelerate fibroblast migration and proliferation. Materials and methodsFibroblasts were cultured in serum-free conditioned media from EGF-MSC; RT-PCR was performed to detect expression of EGF gene in EGF-MSCs. EGF protein levels in cell culture supernatants from EGF-MSC were assayed by ELISA and proliferation of EGF-MSC-treated fibroblasts was performed using MTT assay. Effects of EGF-MSC on fibroblast migration were evaluated using scratch wound and transmigration assays. Cell adhesion molecules, cell dynamics molecules and phospho-(Ser) kinase substrate expressions of EGF-MSC-treated fibroblasts were evaluated by western blotting. ResultsEGF gene expression increased in EGF-MSCs and viability of EGF-MSC-treated fibroblasts was elevated. EGF-MSC-treated fibroblasts showed increased migration compared to controls. Expressions of cell adhesion molecules (-catenin, N-cadherin), cell dynamics molecules (cofilin, ezrin) and phospho-(Ser) kinase substrates (phospho-MAPK/CDK substrate, phospho-Arg-(Ser)-X-Tyr/Phe-X-pSer motif) increased in EGF-MSC-treated fibroblasts. These results imply that EGF-MSCs contributed to enhancing the wound healing process by increased cell adhesion, dynamic effects, fibroblast migration, and proliferation. ConclusionsThis study indicates that EGF-MSCs had a positive influence on fibroblast migration and proliferation and EGF-MSC may provide a useful strategy for wound healing. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | WILEY | - |
dc.relation.isPartOf | CELL PROLIFERATION | - |
dc.subject | SKIN | - |
dc.subject | WOUNDS | - |
dc.subject | DIFFERENTIATION | - |
dc.subject | ANGIOGENESIS | - |
dc.subject | REGENERATION | - |
dc.subject | INDUCTION | - |
dc.subject | MURINE | - |
dc.subject | BURN | - |
dc.title | Effects of human epidermal growth factor gene-transfected mesenchymal stem cells on fibroblast migration and proliferation | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000325923500005 | - |
dc.identifier.doi | 10.1111/cpr.12042 | - |
dc.identifier.bibliographicCitation | CELL PROLIFERATION, v.46, no.4, pp.408 - 415 | - |
dc.identifier.scopusid | 2-s2.0-84880693932 | - |
dc.citation.endPage | 415 | - |
dc.citation.startPage | 408 | - |
dc.citation.title | CELL PROLIFERATION | - |
dc.citation.volume | 46 | - |
dc.citation.number | 4 | - |
dc.contributor.affiliatedAuthor | You, D. H. | - |
dc.contributor.affiliatedAuthor | Nam, M. J. | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | SKIN | - |
dc.subject.keywordPlus | WOUNDS | - |
dc.subject.keywordPlus | DIFFERENTIATION | - |
dc.subject.keywordPlus | ANGIOGENESIS | - |
dc.subject.keywordPlus | REGENERATION | - |
dc.subject.keywordPlus | INDUCTION | - |
dc.subject.keywordPlus | MURINE | - |
dc.subject.keywordPlus | BURN | - |
dc.relation.journalResearchArea | Cell Biology | - |
dc.relation.journalWebOfScienceCategory | Cell Biology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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