Effects of human epidermal growth factor gene-transfected mesenchymal stem cells on fibroblast migration and proliferation
- Authors
- You, D. H.; Nam, M. J.
- Issue Date
- Aug-2013
- Publisher
- WILEY
- Citation
- CELL PROLIFERATION, v.46, no.4, pp.408 - 415
- Journal Title
- CELL PROLIFERATION
- Volume
- 46
- Number
- 4
- Start Page
- 408
- End Page
- 415
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14409
- DOI
- 10.1111/cpr.12042
- ISSN
- 0960-7722
- Abstract
- ObjectivesWe were interested in determining whether epidermal growth factor gene-transfected mesenchymal stem cells (EGF-MSC) would accelerate fibroblast migration and proliferation. Materials and methodsFibroblasts were cultured in serum-free conditioned media from EGF-MSC; RT-PCR was performed to detect expression of EGF gene in EGF-MSCs. EGF protein levels in cell culture supernatants from EGF-MSC were assayed by ELISA and proliferation of EGF-MSC-treated fibroblasts was performed using MTT assay. Effects of EGF-MSC on fibroblast migration were evaluated using scratch wound and transmigration assays. Cell adhesion molecules, cell dynamics molecules and phospho-(Ser) kinase substrate expressions of EGF-MSC-treated fibroblasts were evaluated by western blotting. ResultsEGF gene expression increased in EGF-MSCs and viability of EGF-MSC-treated fibroblasts was elevated. EGF-MSC-treated fibroblasts showed increased migration compared to controls. Expressions of cell adhesion molecules (-catenin, N-cadherin), cell dynamics molecules (cofilin, ezrin) and phospho-(Ser) kinase substrates (phospho-MAPK/CDK substrate, phospho-Arg-(Ser)-X-Tyr/Phe-X-pSer motif) increased in EGF-MSC-treated fibroblasts. These results imply that EGF-MSCs contributed to enhancing the wound healing process by increased cell adhesion, dynamic effects, fibroblast migration, and proliferation. ConclusionsThis study indicates that EGF-MSCs had a positive influence on fibroblast migration and proliferation and EGF-MSC may provide a useful strategy for wound healing.
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