Neuronal damage and gliosis in the somatosensory cortex induced by various durations of transient cerebral ischemia in gerbils
DC Field | Value | Language |
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dc.contributor.author | Lee, Jae-Chul | - |
dc.contributor.author | Ahn, Ji Hyeon | - |
dc.contributor.author | Lee, Dae Hwan | - |
dc.contributor.author | Yan, Bing Chun | - |
dc.contributor.author | Park, Joon Ha | - |
dc.contributor.author | Kim, In Hye | - |
dc.contributor.author | Cho, Geum-Sil | - |
dc.contributor.author | Kim, Young-Myeong | - |
dc.contributor.author | Lee, Bonghee | - |
dc.contributor.author | Park, Chan Woo | - |
dc.contributor.author | Cho, Jun Hwi | - |
dc.contributor.author | Lee, Hui Young | - |
dc.contributor.author | Won, Moo-Ho | - |
dc.date.available | 2020-02-28T23:46:34Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2013-05-13 | - |
dc.identifier.issn | 0006-8993 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14542 | - |
dc.description.abstract | Although many studies regarding ischemic brain damage in the gerbil have been reported, studies on neuronal damage according to various durations of ischemia-reperfusion (I-R) have been limited. In this study, we examined neuronal damage/death and glial changes in the somatosensory cortex 4 days after 5, 10 and 15 min of transient cerebral ischemia using the gerbil. To examine neuronal damage, we used Fluoro-jade B (F-J B, a marker for neuronal degeneration) histofluorescence staining as well as cresyl violet (CV) staining and neuronal nuclei (NeuN, neuronal marker) immunohistochemistry. In the somatosensory cortex, some CV and NeuN positive ((+)) neurons were slightly decreased only in layers III and VI in the 5 min ischemia-group, and the number of CV+ and NeuN(+) neurons were decreased with longer ischemic time. The F-J B histofluorescence staining showed a clear neuronal damage in layers III and VI, and the number of F-J B+ neurons was increased with time of ischemia-reperfusion: in the 15 min ischemia-group, the number of F-J B+ neurons was much higher in layer III than in layer VI. In addition, we immunohistochemically examined gliosis of astrocytes and microglia using anti-glial fibrillary acidic protein (GFAP) and anti-ionized calcium-binding adapter molecule 1 (Iba-1) antibody, respectively. In the 5 min ischemia-group, GFAP(+) astrocytes and Iba-1(+) microglia were distinctively increased in number, and their immunoreactivity was stronger than that in the sham-group. In the 10 and 15 min ischemia-groups, numbers of GFAP(+) and Iba-1(+) glial cells were much more increased with time of ischemia-reperfusion; in the 15 min ischemia-group, their distribution patterns of GFAP(+) and Iba-1(+) glial cells were similar to those in the 10 min ischemia-group. Our fining indicates that neuronal death/damage and gliosis of astrocytes and microglia were apparently increased with longer time of ischemia-reperfusion. (C) 2013 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | BRAIN RESEARCH | - |
dc.subject | FLUORO-JADE B | - |
dc.subject | MONGOLIAN GERBILS | - |
dc.subject | RAT-BRAIN | - |
dc.subject | SELECTIVE VULNERABILITY | - |
dc.subject | PYRAMIDAL NEURONS | - |
dc.subject | GLOBAL-ISCHEMIA | - |
dc.subject | VISUAL-CORTEX | - |
dc.subject | TIME-COURSE | - |
dc.subject | IN-VITRO | - |
dc.subject | HIPPOCAMPUS | - |
dc.title | Neuronal damage and gliosis in the somatosensory cortex induced by various durations of transient cerebral ischemia in gerbils | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000319304600007 | - |
dc.identifier.doi | 10.1016/j.brainres.2013.03.008 | - |
dc.identifier.bibliographicCitation | BRAIN RESEARCH, v.1510, pp.78 - 88 | - |
dc.identifier.scopusid | 2-s2.0-84876665995 | - |
dc.citation.endPage | 88 | - |
dc.citation.startPage | 78 | - |
dc.citation.title | BRAIN RESEARCH | - |
dc.citation.volume | 1510 | - |
dc.contributor.affiliatedAuthor | Lee, Bonghee | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Ischemia-reperfusion | - |
dc.subject.keywordAuthor | Ischemic duration | - |
dc.subject.keywordAuthor | Cerebral cortex | - |
dc.subject.keywordAuthor | Delayed neuronal death | - |
dc.subject.keywordAuthor | Astrocytes | - |
dc.subject.keywordAuthor | Microglia | - |
dc.subject.keywordPlus | FLUORO-JADE B | - |
dc.subject.keywordPlus | MONGOLIAN GERBILS | - |
dc.subject.keywordPlus | RAT-BRAIN | - |
dc.subject.keywordPlus | SELECTIVE VULNERABILITY | - |
dc.subject.keywordPlus | PYRAMIDAL NEURONS | - |
dc.subject.keywordPlus | GLOBAL-ISCHEMIA | - |
dc.subject.keywordPlus | VISUAL-CORTEX | - |
dc.subject.keywordPlus | TIME-COURSE | - |
dc.subject.keywordPlus | IN-VITRO | - |
dc.subject.keywordPlus | HIPPOCAMPUS | - |
dc.relation.journalResearchArea | Neurosciences & Neurology | - |
dc.relation.journalWebOfScienceCategory | Neurosciences | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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