Neuronal damage and gliosis in the somatosensory cortex induced by various durations of transient cerebral ischemia in gerbils
- Authors
- Lee, Jae-Chul; Ahn, Ji Hyeon; Lee, Dae Hwan; Yan, Bing Chun; Park, Joon Ha; Kim, In Hye; Cho, Geum-Sil; Kim, Young-Myeong; Lee, Bonghee; Park, Chan Woo; Cho, Jun Hwi; Lee, Hui Young; Won, Moo-Ho
- Issue Date
- 13-May-2013
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Ischemia-reperfusion; Ischemic duration; Cerebral cortex; Delayed neuronal death; Astrocytes; Microglia
- Citation
- BRAIN RESEARCH, v.1510, pp.78 - 88
- Journal Title
- BRAIN RESEARCH
- Volume
- 1510
- Start Page
- 78
- End Page
- 88
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14542
- DOI
- 10.1016/j.brainres.2013.03.008
- ISSN
- 0006-8993
- Abstract
- Although many studies regarding ischemic brain damage in the gerbil have been reported, studies on neuronal damage according to various durations of ischemia-reperfusion (I-R) have been limited. In this study, we examined neuronal damage/death and glial changes in the somatosensory cortex 4 days after 5, 10 and 15 min of transient cerebral ischemia using the gerbil. To examine neuronal damage, we used Fluoro-jade B (F-J B, a marker for neuronal degeneration) histofluorescence staining as well as cresyl violet (CV) staining and neuronal nuclei (NeuN, neuronal marker) immunohistochemistry. In the somatosensory cortex, some CV and NeuN positive ((+)) neurons were slightly decreased only in layers III and VI in the 5 min ischemia-group, and the number of CV+ and NeuN(+) neurons were decreased with longer ischemic time. The F-J B histofluorescence staining showed a clear neuronal damage in layers III and VI, and the number of F-J B+ neurons was increased with time of ischemia-reperfusion: in the 15 min ischemia-group, the number of F-J B+ neurons was much higher in layer III than in layer VI. In addition, we immunohistochemically examined gliosis of astrocytes and microglia using anti-glial fibrillary acidic protein (GFAP) and anti-ionized calcium-binding adapter molecule 1 (Iba-1) antibody, respectively. In the 5 min ischemia-group, GFAP(+) astrocytes and Iba-1(+) microglia were distinctively increased in number, and their immunoreactivity was stronger than that in the sham-group. In the 10 and 15 min ischemia-groups, numbers of GFAP(+) and Iba-1(+) glial cells were much more increased with time of ischemia-reperfusion; in the 15 min ischemia-group, their distribution patterns of GFAP(+) and Iba-1(+) glial cells were similar to those in the 10 min ischemia-group. Our fining indicates that neuronal death/damage and gliosis of astrocytes and microglia were apparently increased with longer time of ischemia-reperfusion. (C) 2013 Elsevier B.V. All rights reserved.
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