Avenanthramide C from germinated oats exhibits anti-allergic inflammatory effects in mast cells
- Authors
- Dhakal, Hima; Yang, Eun-Ju; Lee, Soyoung; Kim, Min-Jong; Baek, Moon-Chang; Lee, Byungheon; Park, Pil-Hoon; Kwon, Taeg Kyu; Khang, Dongwoo; Song, Kyung-Sik; Kim, Sang-Hyun
- Issue Date
- 3-May-2019
- Publisher
- NATURE PUBLISHING GROUP
- Citation
- SCIENTIFIC REPORTS, v.9
- Journal Title
- SCIENTIFIC REPORTS
- Volume
- 9
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1470
- DOI
- 10.1038/s41598-019-43412-2
- ISSN
- 2045-2322
- Abstract
- Mast cells play a crucial role in allergic diseases via the release of inflammatory mediators, particularly histamine and pro-inflammatory cytokines. Avenanthramide (Avn) C, a polyphenol found mainly in oats, is known to exhibit various biological properties. In this study, we aimed to evaluate the effectiveness of Avn C from germinated oats against mast cell-mediated allergic inflammation. For the in vitro study, RBL-2H3, mouse bone marrow-derived mast cells and rat peritoneal mast cells were used. Avn C (1-100 nM) inhibited the immunoglobulin (Ig) E-stimulated mast cells degranulation by suppressing phosphorylation of phosphoinositide 3-kinase and phospholipase C gamma 1 and decreasing intracellular calcium levels. It inhibited IgE-stimulated secretion of inflammatory cytokines via suppression of FceRI-mediated signaling proteins Lyn, Syk, Akt, and nuclear factor-kappa B. To verify the effects of Avn C in vivo, ovalbumin-induced active systemic anaphylaxis (ASA) and IgE-mediated passive cutaneous anaphylaxis (PCA) models were used. Oral administration of Avn C dose-dependently attenuated the ASA reactions, as evidenced by the inhibition of hypothermia and reduction of elevated serum histamine, IgE, and interleukin-4 levels. Avn C also inhibited the PCA reactions, such as ear swelling and plasma extravasation. Our results suggested that Avn C from germinated oats might be a possible therapeutic candidate for mast cell-mediated allergic inflammation.
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