Dynamic imaging of enzymatic events at polyelectrolyte-disrupted phospholipid membranes using liquid crystals
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Hu, Qiong-Zheng | - |
dc.contributor.author | Shin, Hyunkyung | - |
dc.contributor.author | Jang, Chang-Hyun | - |
dc.date.available | 2020-02-29T00:45:47Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2013-01-01 | - |
dc.identifier.issn | 0267-8292 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14816 | - |
dc.description.abstract | In this study, we employed a simple liquid crystal (LC)-based system to dynamically image enzymatic events at the aqueous/LC interface decorated with polyelectrolyte-disrupted phospholipid membranes. Since polyelectrolytes were shown to disrupt the arrangement of the self-assembled phospholipid monolayer and induced a dark-to-bright shift in the optical response of LCs that support the phospholipid membrane, we observed that the transfer of an aqueous solution of protease onto the polyelectrolyte-disrupted phospholipid membrane resulted in a gradual recovery of the optical response of LCs from bright to dark appearance. Due to the enzymatic event that occurs at the aqueous/LC interface, the generated polyelectrolyte fragments desorbed from the interface to the bulk solution. This led to the restoration of the disrupted phospholipid monolayer, which resulted in recovery of the optical response. These results suggest that the polyelectrolyte-decorated membrane-supported LCs could be potentially used to examine a range of biological interactions that involve polyelectrolytes. Furthermore, the LC-based system holds great promise for label-free and real-time investigation and detection of biomolecular interactions coupled to membrane disruption and restoration, which might have potential utility in the clinical diagnosis and treatment of membrane-associated disease. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | TAYLOR & FRANCIS LTD | - |
dc.relation.isPartOf | LIQUID CRYSTALS | - |
dc.subject | ORDERING TRANSITIONS | - |
dc.subject | AQUEOUS PHASES | - |
dc.subject | SURFACES | - |
dc.subject | INTERFACES | - |
dc.subject | RECOGNITION | - |
dc.subject | MONOLAYERS | - |
dc.subject | BACTERIA | - |
dc.subject | ANTIBODY | - |
dc.subject | BINDING | - |
dc.subject | EPITOPE | - |
dc.title | Dynamic imaging of enzymatic events at polyelectrolyte-disrupted phospholipid membranes using liquid crystals | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000313417600013 | - |
dc.identifier.doi | 10.1080/02678292.2012.733428 | - |
dc.identifier.bibliographicCitation | LIQUID CRYSTALS, v.40, no.1, pp.106 - 111 | - |
dc.identifier.scopusid | 2-s2.0-84872312973 | - |
dc.citation.endPage | 111 | - |
dc.citation.startPage | 106 | - |
dc.citation.title | LIQUID CRYSTALS | - |
dc.citation.volume | 40 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Hu, Qiong-Zheng | - |
dc.contributor.affiliatedAuthor | Shin, Hyunkyung | - |
dc.contributor.affiliatedAuthor | Jang, Chang-Hyun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | liquid crystals | - |
dc.subject.keywordAuthor | sensors | - |
dc.subject.keywordAuthor | enzymatic events | - |
dc.subject.keywordAuthor | polyelectrolytes | - |
dc.subject.keywordAuthor | phospholipid membranes | - |
dc.subject.keywordPlus | ORDERING TRANSITIONS | - |
dc.subject.keywordPlus | AQUEOUS PHASES | - |
dc.subject.keywordPlus | SURFACES | - |
dc.subject.keywordPlus | INTERFACES | - |
dc.subject.keywordPlus | RECOGNITION | - |
dc.subject.keywordPlus | MONOLAYERS | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | ANTIBODY | - |
dc.subject.keywordPlus | BINDING | - |
dc.subject.keywordPlus | EPITOPE | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Crystallography | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.relation.journalWebOfScienceCategory | Crystallography | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.