Dynamic imaging of enzymatic events at polyelectrolyte-disrupted phospholipid membranes using liquid crystals
- Authors
- Hu, Qiong-Zheng; Shin, Hyunkyung; Jang, Chang-Hyun
- Issue Date
- 1-Jan-2013
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- liquid crystals; sensors; enzymatic events; polyelectrolytes; phospholipid membranes
- Citation
- LIQUID CRYSTALS, v.40, no.1, pp.106 - 111
- Journal Title
- LIQUID CRYSTALS
- Volume
- 40
- Number
- 1
- Start Page
- 106
- End Page
- 111
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/14816
- DOI
- 10.1080/02678292.2012.733428
- ISSN
- 0267-8292
- Abstract
- In this study, we employed a simple liquid crystal (LC)-based system to dynamically image enzymatic events at the aqueous/LC interface decorated with polyelectrolyte-disrupted phospholipid membranes. Since polyelectrolytes were shown to disrupt the arrangement of the self-assembled phospholipid monolayer and induced a dark-to-bright shift in the optical response of LCs that support the phospholipid membrane, we observed that the transfer of an aqueous solution of protease onto the polyelectrolyte-disrupted phospholipid membrane resulted in a gradual recovery of the optical response of LCs from bright to dark appearance. Due to the enzymatic event that occurs at the aqueous/LC interface, the generated polyelectrolyte fragments desorbed from the interface to the bulk solution. This led to the restoration of the disrupted phospholipid monolayer, which resulted in recovery of the optical response. These results suggest that the polyelectrolyte-decorated membrane-supported LCs could be potentially used to examine a range of biological interactions that involve polyelectrolytes. Furthermore, the LC-based system holds great promise for label-free and real-time investigation and detection of biomolecular interactions coupled to membrane disruption and restoration, which might have potential utility in the clinical diagnosis and treatment of membrane-associated disease.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 나노화학과 > 1. Journal Articles
- 경영대학 > 금융수학과 > 1. Journal Articles
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.