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Effect of Molecular Weight of Chitosan on Microbial Growth and γ-cyclodextrin Production from Bacillus cereus GU-02
- Issue Date
- 2013
- Publisher
- 한국키틴키토산학회
- Keywords
- Chitosan; Molecular weight; Bacillus sp.; Gamma-cyclodextrin (γ-CD); Antibacterial activity
- Citation
- Journal of Chitin and Chitosan, v.18, no.1, pp.7 - 12
- Journal Title
- Journal of Chitin and Chitosan
- Volume
- 18
- Number
- 1
- Start Page
- 7
- End Page
- 12
- ISSN
- 1229-4160
- Abstract
- Effects of chitosan with different molecular weight on gamma-cyclodextrin (γ-CD) production and bacterial cell growth of Bacillus cereus GU-2 was elucidated. Optimum pH and temperature for bacterial growth and γ-CD production were estimated to be pH 8~10 and 37oC, respectively. Interestingly, among bioactive substances chitosan showed molecular weight dependentinhibitory effect (over 80%) on growth of B. cereus GU-2. Among, MMWC-2 (3,500 Da) revealed the highest (92%) inhibitory effect on bacterial cell growth, considerable as a limiting factor. Consequently, no significant inhibitory effect of both high molecular weight chitosan (HMWC, 200 kDa) and MMWCs on the production of γ-CD was observed. These results made us to hypothesize that γ-CD synthesized by B. cereus GU-2 may be a key modulator involved in bacterial cell viability.
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Related Researcher
- Park, Jae Kweon
- BioNano Technology (Department of Life Sciences)