Orally administered aqueous extract of Inonotus obliquus ameliorates acute inflammation in dextran sulfate sodium (DSS)-induced colitis in mice
DC Field | Value | Language |
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dc.contributor.author | Mishra, Siddhartha Kumar | - |
dc.contributor.author | Kang, Ju Hee | - |
dc.contributor.author | Kim, Dong-Kyu | - |
dc.contributor.author | Oh, Seung Hyun | - |
dc.contributor.author | Kim, Mi Kyung | - |
dc.date.available | 2020-02-29T05:43:10Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2012-09-28 | - |
dc.identifier.issn | 0378-8741 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16160 | - |
dc.description.abstract | Ethnopharmacological relevance: Chaga mushroom (Inonotus obliquus) has been used in folk medicine to treat several disorders through its various biological functions. I. obliquus is claimed to produce general immune-potentiating and strengthening, antiinflammatory, and antitumor properties, but its effects on intestinal inflammation (ulcerative colitis) are clearly not understood. Aim of the study: To determine the effects and mode of action of an aqueous extract of I. obliquus (IOAE) on experimental colitis in mice induced by dextran sulfate sodium (DSS). Materials and methods: Female 5-week-057BL/6 mice were randomized into groups differing in treatment conditions (prevention and treatment) and doses of IOAE (50 and 100 mg/kg body weight). Mice were exposed to DSS (2%) in their drinking water over 7 day to induce acute intestinal inflammation. In colon tissues, we evaluated histological changes by hematoxylin and eosin staining, levels of iNOS by immuno-histochemical staining, and neutrophil influx by myeloperoxidase assay. mRNA expression of pro-inflammatory mediators TNF-alpha, IL-1 beta, IL-6, and IFN-gamma was determined by RT-PCR. Results: Histological examinations indicated that IOAE suppressed edema, mucosal damage, and the loss of crypts induced by DSS. IOAE markedly attenuated DSS-induced iNOS levels and myeloperoxidase accumulation in colon tissues, demonstrating its suppressive effect on infiltration of immune cells. In addition, IOAE significantly inhibited mRNA expression of pro-inflammatory cytokines induced by DSS in colon tissues. Conclusion: Our results suggest anti-inflammatory effect of IOAE at colorectal sites due to down-regulation of the expression of inflammatory mediators. Suppression of TNF-alpha and iNOS together with IL-1 beta by IOAE denotes that it might be a useful supplement in the setting of inflammatory bowel disease. (C) 2012 Elsevier Ireland Ltd. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER IRELAND LTD | - |
dc.relation.isPartOf | JOURNAL OF ETHNOPHARMACOLOGY | - |
dc.subject | NITRIC-OXIDE SYNTHASE | - |
dc.subject | BOWEL-DISEASE | - |
dc.subject | INTESTINAL INFLAMMATION | - |
dc.subject | MEDICINAL MUSHROOM | - |
dc.subject | DRUG-THERAPY | - |
dc.subject | SUPPRESSION | - |
dc.subject | ALPHA | - |
dc.subject | CELLS | - |
dc.subject | MODEL | - |
dc.subject | MYELOPEROXIDASE | - |
dc.title | Orally administered aqueous extract of Inonotus obliquus ameliorates acute inflammation in dextran sulfate sodium (DSS)-induced colitis in mice | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000309485000017 | - |
dc.identifier.doi | 10.1016/j.jep.2012.07.008 | - |
dc.identifier.bibliographicCitation | JOURNAL OF ETHNOPHARMACOLOGY, v.143, no.2, pp.524 - 532 | - |
dc.identifier.scopusid | 2-s2.0-84866045274 | - |
dc.citation.endPage | 532 | - |
dc.citation.startPage | 524 | - |
dc.citation.title | JOURNAL OF ETHNOPHARMACOLOGY | - |
dc.citation.volume | 143 | - |
dc.citation.number | 2 | - |
dc.contributor.affiliatedAuthor | Oh, Seung Hyun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Anti-inflammatory | - |
dc.subject.keywordAuthor | Cytokines suppression | - |
dc.subject.keywordAuthor | Experimental colitis | - |
dc.subject.keywordAuthor | Inonotus obliquus | - |
dc.subject.keywordPlus | NITRIC-OXIDE SYNTHASE | - |
dc.subject.keywordPlus | BOWEL-DISEASE | - |
dc.subject.keywordPlus | INTESTINAL INFLAMMATION | - |
dc.subject.keywordPlus | MEDICINAL MUSHROOM | - |
dc.subject.keywordPlus | DRUG-THERAPY | - |
dc.subject.keywordPlus | SUPPRESSION | - |
dc.subject.keywordPlus | ALPHA | - |
dc.subject.keywordPlus | CELLS | - |
dc.subject.keywordPlus | MODEL | - |
dc.subject.keywordPlus | MYELOPEROXIDASE | - |
dc.relation.journalResearchArea | Plant Sciences | - |
dc.relation.journalResearchArea | Pharmacology & Pharmacy | - |
dc.relation.journalResearchArea | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Plant Sciences | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Medicinal | - |
dc.relation.journalWebOfScienceCategory | Integrative & Complementary Medicine | - |
dc.relation.journalWebOfScienceCategory | Pharmacology & Pharmacy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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