Orally administered aqueous extract of Inonotus obliquus ameliorates acute inflammation in dextran sulfate sodium (DSS)-induced colitis in mice
- Authors
- Mishra, Siddhartha Kumar; Kang, Ju Hee; Kim, Dong-Kyu; Oh, Seung Hyun; Kim, Mi Kyung
- Issue Date
- 28-Sep-2012
- Publisher
- ELSEVIER IRELAND LTD
- Keywords
- Anti-inflammatory; Cytokines suppression; Experimental colitis; Inonotus obliquus
- Citation
- JOURNAL OF ETHNOPHARMACOLOGY, v.143, no.2, pp.524 - 532
- Journal Title
- JOURNAL OF ETHNOPHARMACOLOGY
- Volume
- 143
- Number
- 2
- Start Page
- 524
- End Page
- 532
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16160
- DOI
- 10.1016/j.jep.2012.07.008
- ISSN
- 0378-8741
- Abstract
- Ethnopharmacological relevance: Chaga mushroom (Inonotus obliquus) has been used in folk medicine to treat several disorders through its various biological functions. I. obliquus is claimed to produce general immune-potentiating and strengthening, antiinflammatory, and antitumor properties, but its effects on intestinal inflammation (ulcerative colitis) are clearly not understood. Aim of the study: To determine the effects and mode of action of an aqueous extract of I. obliquus (IOAE) on experimental colitis in mice induced by dextran sulfate sodium (DSS). Materials and methods: Female 5-week-057BL/6 mice were randomized into groups differing in treatment conditions (prevention and treatment) and doses of IOAE (50 and 100 mg/kg body weight). Mice were exposed to DSS (2%) in their drinking water over 7 day to induce acute intestinal inflammation. In colon tissues, we evaluated histological changes by hematoxylin and eosin staining, levels of iNOS by immuno-histochemical staining, and neutrophil influx by myeloperoxidase assay. mRNA expression of pro-inflammatory mediators TNF-alpha, IL-1 beta, IL-6, and IFN-gamma was determined by RT-PCR. Results: Histological examinations indicated that IOAE suppressed edema, mucosal damage, and the loss of crypts induced by DSS. IOAE markedly attenuated DSS-induced iNOS levels and myeloperoxidase accumulation in colon tissues, demonstrating its suppressive effect on infiltration of immune cells. In addition, IOAE significantly inhibited mRNA expression of pro-inflammatory cytokines induced by DSS in colon tissues. Conclusion: Our results suggest anti-inflammatory effect of IOAE at colorectal sites due to down-regulation of the expression of inflammatory mediators. Suppression of TNF-alpha and iNOS together with IL-1 beta by IOAE denotes that it might be a useful supplement in the setting of inflammatory bowel disease. (C) 2012 Elsevier Ireland Ltd. All rights reserved.
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