Enhanced delivery of T cells to tumor after chemotherapy using membrane-anchored, apoptosis-targeted peptide
- Authors
- He, Xiaofeng; Bonaparte, Napolean; Kim, Soyoun; Acharya, Bodhraj; Lee, Ji-Young; Chi, Lianhua; Lee, Hyoung-Joo; Paik, Young-Ki; Moon, Pyong-Gon; Baek, Moon-Chang; Lee, Eun-Kyu; Kim, Jong-Ho; Kim, In-San; Lee, Byung-Heon
- Issue Date
- Sep-2012
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Biological anchor for membrane; Chemotherapy; Apoptosis; Peptide; T cell delivery
- Citation
- JOURNAL OF CONTROLLED RELEASE, v.162, no.3, pp.521 - 528
- Journal Title
- JOURNAL OF CONTROLLED RELEASE
- Volume
- 162
- Number
- 3
- Start Page
- 521
- End Page
- 528
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16161
- DOI
- 10.1016/j.jconrel.2012.07.023
- ISSN
- 0168-3659
- Abstract
- Chemotherapy-induced apoptosis of tumor cells enhances the antigen presentation and sensitizes tumor cells to T cell-mediated cytotoxicity. Here we harnessed the apoptosis of tumor cells as a homing signal for the delivery of T cells to tumor. Jurkat T cells were anchored with ApoPep-1, an apoptosis-targeted peptide ligand, using the biocompatible anchor for membrane (BAM), an oleyl acid derivative. The ApoPep-1-BAM conjugate was efficiently anchored to cell membrane, while little anchoring was obtained with ApoPep-1 alone. The retention period of the ApoPep-1-BAM conjugate on cell membrane was approximately 80 and 40 min in the absence and presence of serum, respectively. ApoPep-1 was resistant to degradation in serum until 2 h. The apoptosis-targeted T cells that were anchored with the ApoPep-1-BAM preferentially bound to apoptotic tumor cells over living cells. When intravenously injected into tumor-bearing mice, the number of apoptosis-targeted T cells and in vivo fluorescence signals by the homing of the cells to doxorubicin-treated tumor were higher than those of untargeted T cells. Accumulation of apoptosis-targeted T cells at other organs such as liver was not detected. These results suggest that the chemotherapy-induced apoptosis and subsequent enhancement of T cell delivery to tumor by the membrane anchoring of the apoptosis-targeted peptide could be a novel strategy for cancer immunotherapy. (C) 2012 Elsevier B. V. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - ETC > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16161)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.