Effect of Anti-Siglec-F Antibody and Reactive Oxygen Species Blocking on Histamine Release in Urinary Bladder of Ovalbumin-Treated Mice
- Authors
- Choi, Bo-Hwa; Choo, Gwoan-Youb; Kang, Ju-Hee; Lee, Choong-Yeol; Park, Chang-Shin
- Issue Date
- Sep-2012
- Publisher
- KOREAN CONTINENCE SOC
- Keywords
- Histamine; Mice; Ovalbumin; Reactive oxygen species; Sialic acid binding Ig-like lectin; Urinary bladder
- Citation
- INTERNATIONAL NEUROUROLOGY JOURNAL, v.16, no.3, pp.122 - 125
- Journal Title
- INTERNATIONAL NEUROUROLOGY JOURNAL
- Volume
- 16
- Number
- 3
- Start Page
- 122
- End Page
- 125
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16185
- DOI
- 10.5213/inj.2012.16.3.122
- ISSN
- 2093-4777
- Abstract
- Purpose: Sialic acid-binding Ig-like lectin (Siglec) is an immune inhibitory receptor that plays a role in the negative regulation of the activation of immune cells. This study aimed to evaluate the effects of anti-Siglec-F on plasma and urinary histamine levels in ovalbumin (OVA)-challenged urinary bladder in mice. Methods: Thirty BALB/c mice were used. In group I (control group, n = 5), mice were sensitized with OVA and challenged with saline. In group II (OVA challenge group, n = 5), OVA was used for intraperitoneal sensitization and intravesical challenge. The challenged mice in group III (control immunoglobulin G [IgG] group, n = 5) and those in group IV (anti-Siglec-F group, n = 5) were intraperitoneally pretreated with rabbit control IgG or anti-Siglec-F antibody, respectively. In groups V (N-acetylcysteine [NAC] in OVA challenge group, n = 5) and VI (control NAC only, n = 5), mice were pretreated with NAC. Results: Urinary histamine concentrations were significantly higher 7 days after intravesical OVA challenge (P < 0.01), whereas plasma histamine levels were not. Pretreatment with anti-Siglec-F antibody significantly prevented the increase in urinary histamine release (P < 0.05), whereas pretreatment with the IgG antibody control did not. Also, pretreatment of the OVA challenge group with NAC did not affect the histamine concentration in either urine or plasma. Conclusions: Systemic anti-Siglec-F treatment showed anti-allergic effects at least on local histamine release, particularly in the lower urinary bladder.
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