Asian Sand Dust Enhances Allergen-Induced Th2 Allergic Inflammatory Changes and Mucin Production in BALB/c Mouse Lungs
- Authors
- Kang, Il Gyu; Jung, Joo Hyun; Kim, Seon Tae
- Issue Date
- Jul-2012
- Publisher
- KOREAN ACAD ASTHMA ALLERGY & CLINICAL IMMUNOLOGY
- Keywords
- Asian sand dust; airway inflammation; mucin
- Citation
- ALLERGY ASTHMA & IMMUNOLOGY RESEARCH, v.4, no.4, pp.206 - 213
- Journal Title
- ALLERGY ASTHMA & IMMUNOLOGY RESEARCH
- Volume
- 4
- Number
- 4
- Start Page
- 206
- End Page
- 213
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16309
- DOI
- 10.4168/aair.2012.4.4.206
- ISSN
- 2092-7355
- Abstract
- Purpose: Recent studies have reported that Asian sand dust (ASD) has a potential risk of aggravating airway inflammation. The purpose of this study was to investigate the effect of ASD on inflammation and mucin production in the airways of allergic mice. Methods: Forty BALB/c female mice were divided into four groups: saline (group 1); ASD (group 2); ovalbumin (OVA) alone (group 3); and OVA+ASD (group 4). OVA-specific immunoglobulin E (IgE) in serum and interleukin (IL)-4, IL-5, IL-13, and interferon-gamma (IFN-gamma) in bronchoalveolar lavage fluid (BALF) were measured by enzyme-linked immunosorbent assay (ELISA). Hematoxylin & eosin (H&E) and Periodic acid-Schiff (PAS) staining was performed on lung tissues. In addition, immunohistochemical staining for IL-4, IL-5, MUC5AC, and transforming growth factor alpha (TOE-alpha) was conducted. Results: Serum IgE levels were significantly higher in group 4 than in group 3 (P<0.05). IL-4 and IL-5 in BALF were significantly higher in group 4 than in group 3 (P<0.05, respectively). Based on H&E staining, inflammatory cell numbers were significantly greater in group 4 than in the other groups (P<0.05(. The number of PAS-positive cells was also significantly greater in groups 3 and 4 than in groups 1 and 2 (P<0.05). The numbers of IL-4 and IL-5-positive cells were higher in group 4 than in group 3 (P<0.05). The number of MUC5AC and TGF-alpha-positive cells were also higher in group 4 than in group 3 (P<0.05). Conclusions: Our data suggest that ASD increases cytokine expression and mucin production in an allergic murine model. The increased inflammatory reactions were related to cytokine production.
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