Measuring ligand-receptor binding events on polymeric surfaces with periodic wave patterns using liquid crystals
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Han, Gyeo-Re | - |
dc.contributor.author | Jang, Chang-Hyun | - |
dc.date.available | 2020-02-29T05:47:09Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2012-06-01 | - |
dc.identifier.issn | 0927-7765 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16332 | - |
dc.description.abstract | In this study, we measured ligand-receptor binding events on polymeric substrates with periodic nanostructures using liquid crystals (LCs). Periodic sinusoidal wave patterns were generated through the buckling of the poly-(dimethylsiloxane) (PDMS) substrate on a cylindrical surface followed by replicating the associated relief structures on a poly-(urethane acrylate) (PUA) surface, where a film of gold was deposited. Avidin was then covalently immobilized onto a gold surface decorated with carboxylic acid-terminated self-assembled monolayer via NHS/EDC chemistry. The optical response of the device showed that the orientation of nematic 4-cyano-4'-pentylbiphenyl (5CB) was parallel to the plane of the avidin surface. However, the formation of the avidin-biotin complexes disturbed the sinusoidal topographies of the surface, and induced a random orientation of LCs, which produced a distinctive change in the optical response. We also confirmed the specific ligand-receptor interactions using ellipsometry and atomic force microscopy (AFM). These results suggest that polymeric surfaces with continuous wavy features could be used to develop novel LC-based sensors for the detection of ligand-receptor binding events. (C) 2012 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER SCIENCE BV | - |
dc.relation.isPartOf | COLLOIDS AND SURFACES B-BIOINTERFACES | - |
dc.subject | NANOMETER-SCALE TOPOGRAPHY | - |
dc.subject | PLASMON RESONANCE | - |
dc.subject | PROTEINS | - |
dc.subject | ORIENTATIONS | - |
dc.subject | FILMS | - |
dc.subject | GOLD | - |
dc.title | Measuring ligand-receptor binding events on polymeric surfaces with periodic wave patterns using liquid crystals | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000302979900013 | - |
dc.identifier.doi | 10.1016/j.colsurfb.2012.01.023 | - |
dc.identifier.bibliographicCitation | COLLOIDS AND SURFACES B-BIOINTERFACES, v.94, pp.89 - 94 | - |
dc.identifier.scopusid | 2-s2.0-84859157632 | - |
dc.citation.endPage | 94 | - |
dc.citation.startPage | 89 | - |
dc.citation.title | COLLOIDS AND SURFACES B-BIOINTERFACES | - |
dc.citation.volume | 94 | - |
dc.contributor.affiliatedAuthor | Han, Gyeo-Re | - |
dc.contributor.affiliatedAuthor | Jang, Chang-Hyun | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Liquid crystals | - |
dc.subject.keywordAuthor | 4-Cyano-4 &apos | - |
dc.subject.keywordAuthor | -pentylbiphenyl (5CB) | - |
dc.subject.keywordAuthor | Polymeric substrates | - |
dc.subject.keywordAuthor | Buckling | - |
dc.subject.keywordAuthor | Ligand-receptor binding | - |
dc.subject.keywordAuthor | Sensors | - |
dc.subject.keywordPlus | NANOMETER-SCALE TOPOGRAPHY | - |
dc.subject.keywordPlus | PLASMON RESONANCE | - |
dc.subject.keywordPlus | PROTEINS | - |
dc.subject.keywordPlus | ORIENTATIONS | - |
dc.subject.keywordPlus | FILMS | - |
dc.subject.keywordPlus | GOLD | - |
dc.relation.journalResearchArea | Biophysics | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Materials Science | - |
dc.relation.journalWebOfScienceCategory | Biophysics | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.relation.journalWebOfScienceCategory | Materials Science, Biomaterials | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.