Measuring ligand-receptor binding events on polymeric surfaces with periodic wave patterns using liquid crystals
- Authors
- Han, Gyeo-Re; Jang, Chang-Hyun
- Issue Date
- 1-Jun-2012
- Publisher
- ELSEVIER SCIENCE BV
- Keywords
- Liquid crystals; 4-Cyano-4 ' -pentylbiphenyl (5CB); Polymeric substrates; Buckling; Ligand-receptor binding; Sensors
- Citation
- COLLOIDS AND SURFACES B-BIOINTERFACES, v.94, pp.89 - 94
- Journal Title
- COLLOIDS AND SURFACES B-BIOINTERFACES
- Volume
- 94
- Start Page
- 89
- End Page
- 94
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/16332
- DOI
- 10.1016/j.colsurfb.2012.01.023
- ISSN
- 0927-7765
- Abstract
- In this study, we measured ligand-receptor binding events on polymeric substrates with periodic nanostructures using liquid crystals (LCs). Periodic sinusoidal wave patterns were generated through the buckling of the poly-(dimethylsiloxane) (PDMS) substrate on a cylindrical surface followed by replicating the associated relief structures on a poly-(urethane acrylate) (PUA) surface, where a film of gold was deposited. Avidin was then covalently immobilized onto a gold surface decorated with carboxylic acid-terminated self-assembled monolayer via NHS/EDC chemistry. The optical response of the device showed that the orientation of nematic 4-cyano-4'-pentylbiphenyl (5CB) was parallel to the plane of the avidin surface. However, the formation of the avidin-biotin complexes disturbed the sinusoidal topographies of the surface, and induced a random orientation of LCs, which produced a distinctive change in the optical response. We also confirmed the specific ligand-receptor interactions using ellipsometry and atomic force microscopy (AFM). These results suggest that polymeric surfaces with continuous wavy features could be used to develop novel LC-based sensors for the detection of ligand-receptor binding events. (C) 2012 Elsevier B.V. All rights reserved.
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