의이인탕 과립제가 고지방식이로 비만이 유도된 C57BL/6J Mice에 미치는 항염증 및 항비만 효과

Abstract

Objectives :The study was designed to experiment anti-inflammation and anti-obesity effect of Euiiin-tang(yìyǐrén-tāng) granules to the C57 BL/6J mice which was induced obesity by high-fat diet. Methods :We performed a cytotoxicity analysis of Euiiin-tang by MTT assay principles. We investigated the effects of Euiiin-tang on restraining enzyme revelation of inflammation acceleration and hindering inflammation by measuring iNOS, COX-2 and NF-κB revelation. We treated Euiiin-tang to 3T3-L1 in Oil Red O experiment and measured a reduction effect of accumulation of fat. In order to investigate the obese inhibitory effects of Euiiin-tang, C57BL/6 mice were induced by high fat diet. C57BL/6 mice were divided into three group(normal, high fat diet, high fat diet with Euiiin-tang granules) and fed for 6weeks. The measurements have been performed on ; the body weight ; the weight of mice’ liver, kidney and epididymal AT ; the quantity of AST, ALT, γ-GTP, BUN, total cholesterol, HDL, LDL, plasma insulin, blood leptin. Results :After an experiment of measuring iNOS and COX-2 revelation, Euiiin-tang showed efficacy of restraining enzyme revelation of inflammation acceleration. Treating Euiiin-tang hindered vitality of NF-κB, so we could identify effects of hinderance of inflammation. It was shown that accumulation of fat was significantly reduced after treating Euiiin-tang to 3T3-L1 in Oil Red O experiment. After treating Euiiin-tang in oral for 6 weeks’ experiment, it was identified that it had an efficacy of restraining body weight gain. As a result of measuring the weight of mice’ liver, kidney and epididymis, the weights were reduced comparing with high fat diet group, so it came out that it has prevention effect in accumulation of fats. The AST, ALT, γ-GTP, BUN levels were lower in group of Euiiin-tang than that of high fat diet group. In terms of blood lipids, it was verified that total cholesterol level was in normal range with group of Euiiin-tang and HDL cholesterol level was higher than that of the high fat diet group. While concentration of plasma insulin was lower in group of Euiiin-tang than that of high fat diet group. Also the blood leptin concentration was lower in group of Euiiin-tang than that of high fat diet group. Conclusions :From all these results, it can be concluded that the Euiiin-tang showed a significant efficacy as obesity treatment and it is thought to be a safe treatment that we could expect even the efficacy of anti-inflammatory.