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Complex Detection Between Transcription Regulator and Promoter DNA by UV Spectroscopic Method
- Authors
- Kyungmin Lee; 강종백
- Issue Date
- Sep-2012
- Publisher
- 조선대학교 기초과학연구원
- Keywords
- Spectroscopic Method; Melting Temperature; Metalloregulator; Transcription Regulator
- Citation
- 조선자연과학논문집, v.5, no.3, pp.163 - 167
- Journal Title
- 조선자연과학논문집
- Volume
- 5
- Number
- 3
- Start Page
- 163
- End Page
- 167
- ISSN
- 2005-1042
- Abstract
- UV spectrophotometer was used to detect protein-DNA complex from DNA melting profile under constant temperature increase. Melting temperature (Tm) was 43℃ in copA duplex DNA alone. In the presence of Proteus mirabilis transcription regulator protein (PMTR) protein at 0.2 and 0.4 μM, Tm’s were 45 ± 0.5 and 47.6 ± 0.6oC, respectively. According to fluorescence polarization and gel shift assay. PMTR:copA complex was detected by the retarded migration on gel and the dissociation constant (K_d) was (9.2 ± 2.8) × 10^(-9) M.
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Related Researcher
- Gang, Jong Back
- BioNano Technology (Department of Chemistry)