大茴香 물추출물이 마우스 대식세포주(RAW 264.7 cell line)의 hydrogen peroxide 생성에 미치는 영향

Abstract

The purpose of this study is to investigate effects of Anisi stellati Fructus Water Extract on hydrogen peroxide production in RAW 264.7 mouse macrophages. Anisi stellati Fructus were extracted by hot water. Effects of Anisi stellati Fructus water extract (AS) on hydrogen peroxide production in RAW 264.7 were measured by dihydrorhodamine 123 assay after 20, 24, 28, 44, 48, and 52 h incubation at the concentrations of 10, 25, 50, and 100 ㎍/mL. For 20 h incubation, AS significantly increased hydrogen peroxide production in RAW 264.7 cells by 108.6 ± 1.56%, 109.5 ± 1.94%, 108.4 ± 1.14%, and 107.3 ± 3.06% at the concentrations of 10, 25, 50, and 100 ㎍/mL (P < 0.05) respectively. For 24, 28, 44, 48, and 52 h incubation, AS also significantly increased hydrogen peroxide production in RAW 264.7 cells at the concentrations of 10, 25, 50, and 100 ㎍/mL (P < 0.05). These results suggest that Anisi stellati Fructus has the immune - enhancing property related with its increase of hydrogen peroxide production in macrophages.