Liquid crystal-based aptasensor for the detection of interferon-gamma and its application in the diagnosis of tuberculosis using human blood
- Authors
- Kim, Hyeong Jin; Jang, Chang-Hyun
- Issue Date
- 1-Mar-2019
- Publisher
- ELSEVIER SCIENCE SA
- Keywords
- Liquid crystal (LC); 4-cyano-4 ' -pentylbiphenyl (5CB); Interferon-gamma (IFN-gamma); Aptamer; Tuberculosis (TB); Human blood sample
- Citation
- SENSORS AND ACTUATORS B-CHEMICAL, v.282, pp.574 - 579
- Journal Title
- SENSORS AND ACTUATORS B-CHEMICAL
- Volume
- 282
- Start Page
- 574
- End Page
- 579
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/1731
- DOI
- 10.1016/j.snb.2018.11.104
- ISSN
- 0925-4005
- Abstract
- We report the development of a liquid crystal (LC)-based aptasensor for the detection of interferon-gamma (IFN-gamma) in human blood as well as the diagnosis of tuberculosis (TB). In this system, the binding of IFN-gamma to an aptamer immobilized on a surface disrupts the orientation of LCs, inducing a transition from a homeotropic orientation to a random one. This change in the orientation of the LCs can easily be converted and observed as a shift from a dark optical LC image to a bright one under a polarized light microscope. Through this sensing mechanism, IFN-gamma levels as low as 1 pM (17 pg/ml) could be detected. This LC-based aptasensor was employed for the diagnosis of TB using blood samples from patients with latent TB. With this LC-based approach, not only could IFN-gamma readily be detected, but also latent TB could be diagnosed using human blood simply and effectively without any intricate processes or instrumentation. Therefore, our present research provides a promising IFN-gamma sensor with applications in clinical diagnosis of various infectious diseases, and in immunological research.
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