Bench-scale ex situ diesel removal process using a biobarrier and surfactant flushing
- Authors
- 이영철; 우성근; Eun-Sil Choi; 안영희; 박준홍; Myungjin Lee; 양지원
- Issue Date
- May-2012
- Publisher
- 한국공업화학회
- Keywords
- Ex situ biodegradation; Diesel oil; DGGE; T-RFLP; Bench-scale system
- Citation
- Journal of Industrial and Engineering Chemistry, v.18, no.3, pp.882 - 887
- Journal Title
- Journal of Industrial and Engineering Chemistry
- Volume
- 18
- Number
- 3
- Start Page
- 882
- End Page
- 887
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17367
- DOI
- 10.1016/j.jiec.2012.01.020
- ISSN
- 1226-086X
- Abstract
- Ex situ diesel removal was demonstrated using a biobarrier with immobilized cells and surfactant flushing in a bench-scale system. Four strains (two Acinetobacter sp., one Gordonia sp., and one Rhodococcus sp.) isolated from a diesel-contaminated site were immobilized onto a matrix to act as a biofiller. Peat moss, bentonite, and alginate were used as a hybrid support, and a procedure for the use of a bench-scale biobarrier was also employed. According to a microbial counting assay used for the biobarrier, the total amount of bacterial cells increased from approximately 2 109 to 8 109 (colony forming unit (CFU)/g) and the amount of inoculated diesel-degrading bacteria slightly increased from 2 106 to 5 106 (CFU/g) in the same period (over 30 days). This increase resulted in the reduction of diesel from 6000 45 mg/kg to below 5 mg/kg. While 99.9%, i.e. up to below 5 mg/L of the diesel in soil was treated during 30 days of operation, diesel biodegradation accounted for 24.3% of the reduction of diesel.
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