Right triangular prism-shaped poly(Dimethylsiloxane) (PDMS) Microdevice for multiplex PCR employing a single heater
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wu, W. | - |
dc.contributor.author | Trinh, K.T.L. | - |
dc.contributor.author | Lee, N.Y. | - |
dc.date.available | 2020-02-29T09:43:33Z | - |
dc.date.created | 2020-02-11 | - |
dc.date.issued | 2012 | - |
dc.identifier.issn | 0000-0000 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17427 | - |
dc.description.abstract | We propose a right triangular prism-shaped microdevice with serpentine microchannels fabricated on its slanted plane, and apply the device for on-chip flow-through PCR employing a single heater. The inclined nature of the qiandu-shaped microdevice enables the formation of a surface temperature gradient along the slanted plane of the microchannel by the use of a single heater. A 409 bp lung cancer biomarker and a plasmid vector targeting a 230 bp gene fragment were simultaneously amplified in less than 30 min on a single microdevice, paving the way for a microscale, multiplex PCR on a single platform with a single heater. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | Chemical and Biological Microsystems Society | - |
dc.relation.isPartOf | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 | - |
dc.subject | Atmospheric temperature | - |
dc.subject | Multiplexing | - |
dc.subject | Prisms | - |
dc.subject | Serpentine | - |
dc.subject | Thermal gradients | - |
dc.subject | Flowthrough | - |
dc.subject | Micro-device | - |
dc.subject | Multiplex pcr | - |
dc.subject | Right triangular prism-shape | - |
dc.subject | Single heater | - |
dc.subject | Microchannels | - |
dc.title | Right triangular prism-shaped poly(Dimethylsiloxane) (PDMS) Microdevice for multiplex PCR employing a single heater | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.bibliographicCitation | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, pp.1507 - 1509 | - |
dc.identifier.scopusid | 2-s2.0-84901746656 | - |
dc.citation.endPage | 1509 | - |
dc.citation.startPage | 1507 | - |
dc.citation.title | Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012 | - |
dc.contributor.affiliatedAuthor | Wu, W. | - |
dc.contributor.affiliatedAuthor | Trinh, K.T.L. | - |
dc.contributor.affiliatedAuthor | Lee, N.Y. | - |
dc.type.docType | Conference Paper | - |
dc.subject.keywordAuthor | Flow-through PCR | - |
dc.subject.keywordAuthor | Multiplex PCR | - |
dc.subject.keywordAuthor | PDMS microdevice | - |
dc.subject.keywordAuthor | Right triangular prism-shape | - |
dc.subject.keywordAuthor | Single heater | - |
dc.subject.keywordAuthor | Thermal gradient | - |
dc.subject.keywordPlus | Atmospheric temperature | - |
dc.subject.keywordPlus | Multiplexing | - |
dc.subject.keywordPlus | Prisms | - |
dc.subject.keywordPlus | Serpentine | - |
dc.subject.keywordPlus | Thermal gradients | - |
dc.subject.keywordPlus | Flowthrough | - |
dc.subject.keywordPlus | Micro-device | - |
dc.subject.keywordPlus | Multiplex pcr | - |
dc.subject.keywordPlus | Right triangular prism-shape | - |
dc.subject.keywordPlus | Single heater | - |
dc.subject.keywordPlus | Microchannels | - |
dc.description.journalRegisteredClass | scopus | - |
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