Right triangular prism-shaped poly(Dimethylsiloxane) (PDMS) Microdevice for multiplex PCR employing a single heater

Abstract

We propose a right triangular prism-shaped microdevice with serpentine microchannels fabricated on its slanted plane, and apply the device for on-chip flow-through PCR employing a single heater. The inclined nature of the qiandu-shaped microdevice enables the formation of a surface temperature gradient along the slanted plane of the microchannel by the use of a single heater. A 409 bp lung cancer biomarker and a plasmid vector targeting a 230 bp gene fragment were simultaneously amplified in less than 30 min on a single microdevice, paving the way for a microscale, multiplex PCR on a single platform with a single heater.