Right triangular prism-shaped poly(Dimethylsiloxane) (PDMS) Microdevice for multiplex PCR employing a single heater
- Authors
- Wu, W.; Trinh, K.T.L.; Lee, N.Y.
- Issue Date
- 2012
- Publisher
- Chemical and Biological Microsystems Society
- Keywords
- Flow-through PCR; Multiplex PCR; PDMS microdevice; Right triangular prism-shape; Single heater; Thermal gradient
- Citation
- Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012, pp.1507 - 1509
- Journal Title
- Proceedings of the 16th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2012
- Start Page
- 1507
- End Page
- 1509
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17427
- ISSN
- 0000-0000
- Abstract
- We propose a right triangular prism-shaped microdevice with serpentine microchannels fabricated on its slanted plane, and apply the device for on-chip flow-through PCR employing a single heater. The inclined nature of the qiandu-shaped microdevice enables the formation of a surface temperature gradient along the slanted plane of the microchannel by the use of a single heater. A 409 bp lung cancer biomarker and a plasmid vector targeting a 230 bp gene fragment were simultaneously amplified in less than 30 min on a single microdevice, paving the way for a microscale, multiplex PCR on a single platform with a single heater.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 바이오나노학과 > 1. Journal Articles
- 산업·환경대학원 > 산업환경공학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17427)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.