Immobilization of Glucose Oxidase within Chitosan and Sol-Gel Matrix for Biosensors
- Authors
- Lee, Su Yeon; Yoon, Hyon Hee
- Issue Date
- 2012
- Publisher
- TAYLOR & FRANCIS LTD
- Keywords
- Ferrocene; Glucose oxidase; Chitosan; Sol-gel; Glucose sensor
- Citation
- MOLECULAR CRYSTALS AND LIQUID CRYSTALS, v.566, pp.38 - 44
- Journal Title
- MOLECULAR CRYSTALS AND LIQUID CRYSTALS
- Volume
- 566
- Start Page
- 38
- End Page
- 44
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17574
- DOI
- 10.1080/15421406.2012.701116
- ISSN
- 1542-1406
- Abstract
- Enzyme-modified electrodes were fabricated by entrapping glucose oxidase (GOx) and ferrocene (Fc) using chitosan membrane and sol-gel matrix. In chitosan membrane entrapment method, the ferrocene and glucose oxidase were coated sequentially on a glassy carbon electrode and entrapped by chitosan. In sol-gel matrix method, the ferrocene and glucose oxidase were embedded into sol-gel matrix without using any complicated linking processes. The prepared electrode was characterized by cyclic voltammetry(CV). Both Fc/GOx/CHI electrode and Fc/GOx/Sol-gel exhibited a good electrochemical performance for the glucose analysis. The response current of the both Fc/GOx/CHI and Fc/GOx/Sol-gel electrodes increased linearly with the increase of glucose concentration in the range of 0-10 mM glucose. The apparent Michaelis-Menten constants (Km) for the enzymes immobilized in the chitosan membrane and sol-gel matrix were observed to be 1.67 mM and 12.59 mM, respectively. The maximum current of the Fc/GOx/CHI and Fc/GOx/Sol-gel electrodes were 5.85 mu A and 7.52 mu A, respectively.
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