Simultaneous quantification of multiple biomarkers on a self-calibrating microfluidic paper-based analytic device
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Kim, SeJin | - |
dc.contributor.author | Kim, Dami | - |
dc.contributor.author | Kim, Sanghyo | - |
dc.date.available | 2020-03-03T06:42:10Z | - |
dc.date.created | 2020-02-24 | - |
dc.date.issued | 2020-02 | - |
dc.identifier.issn | 0003-2670 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17602 | - |
dc.description.abstract | In this study, we developed a point-of-care assay platform with simultaneous detection and self-calibration capabilities for multiple targets based on a microfluidic paper-based analytical device (mu PAD). This system is easily manufactured using a wax printing method on chromatographic paper. The design pattern consists of a zone of detection and a calibrant zone for controlled loading using wax barriers with different thicknesses. We showed the utility and applicability of this approach by a proofof-concept study for two clinically important markers: glucose and lactate. With the naked eye, the results could be fully distinguished and recorded to evaluate the analytical performance with a flatbed scanner. The detection limits of glucose and lactate were 0.3125 mM and 0.2975 mM, respectively, and simultaneous detection was possible from a small sample (0.4 mu L) with high sensitivity. Furthermore, this device has a self-calibration function, which minimizes the influence of environmental conditions (i.e., ambient light intensity, temperature, humidity, and pressure). Therefore, the developed multiplex paper-based device is promising for clinical multianalyte point-of-care testing since it is easy to manufacture, cost-effective, user-friendly, and highly sensitive. (C) 2019 Elsevier B.V. All rights reserved. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ELSEVIER | - |
dc.relation.isPartOf | ANALYTICA CHIMICA ACTA | - |
dc.title | Simultaneous quantification of multiple biomarkers on a self-calibrating microfluidic paper-based analytic device | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000505562300012 | - |
dc.identifier.doi | 10.1016/j.aca.2019.10.068 | - |
dc.identifier.bibliographicCitation | ANALYTICA CHIMICA ACTA, v.1097, pp.120 - 126 | - |
dc.description.isOpenAccess | N | - |
dc.identifier.scopusid | 2-s2.0-85075396773 | - |
dc.citation.endPage | 126 | - |
dc.citation.startPage | 120 | - |
dc.citation.title | ANALYTICA CHIMICA ACTA | - |
dc.citation.volume | 1097 | - |
dc.contributor.affiliatedAuthor | Kim, SeJin | - |
dc.contributor.affiliatedAuthor | Kim, Dami | - |
dc.contributor.affiliatedAuthor | Kim, Sanghyo | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Colorimetric | - |
dc.subject.keywordAuthor | Glucose | - |
dc.subject.keywordAuthor | Paper-based devices | - |
dc.subject.keywordAuthor | Point-of-care | - |
dc.subject.keywordAuthor | Self-calibration | - |
dc.subject.keywordPlus | COLORIMETRIC DETECTION | - |
dc.subject.keywordPlus | URIC-ACID | - |
dc.subject.keywordPlus | REAL-TIME | - |
dc.subject.keywordPlus | GLUCOSE | - |
dc.subject.keywordPlus | SMARTPHONE | - |
dc.subject.keywordPlus | BIOASSAY | - |
dc.subject.keywordPlus | BLOOD | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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