Simultaneous quantification of multiple biomarkers on a self-calibrating microfluidic paper-based analytic device
- Authors
- Kim, SeJin; Kim, Dami; Kim, Sanghyo
- Issue Date
- Feb-2020
- Publisher
- ELSEVIER
- Keywords
- Colorimetric; Glucose; Paper-based devices; Point-of-care; Self-calibration
- Citation
- ANALYTICA CHIMICA ACTA, v.1097, pp.120 - 126
- Journal Title
- ANALYTICA CHIMICA ACTA
- Volume
- 1097
- Start Page
- 120
- End Page
- 126
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17602
- DOI
- 10.1016/j.aca.2019.10.068
- ISSN
- 0003-2670
- Abstract
- In this study, we developed a point-of-care assay platform with simultaneous detection and self-calibration capabilities for multiple targets based on a microfluidic paper-based analytical device (mu PAD). This system is easily manufactured using a wax printing method on chromatographic paper. The design pattern consists of a zone of detection and a calibrant zone for controlled loading using wax barriers with different thicknesses. We showed the utility and applicability of this approach by a proofof-concept study for two clinically important markers: glucose and lactate. With the naked eye, the results could be fully distinguished and recorded to evaluate the analytical performance with a flatbed scanner. The detection limits of glucose and lactate were 0.3125 mM and 0.2975 mM, respectively, and simultaneous detection was possible from a small sample (0.4 mu L) with high sensitivity. Furthermore, this device has a self-calibration function, which minimizes the influence of environmental conditions (i.e., ambient light intensity, temperature, humidity, and pressure). Therefore, the developed multiplex paper-based device is promising for clinical multianalyte point-of-care testing since it is easy to manufacture, cost-effective, user-friendly, and highly sensitive. (C) 2019 Elsevier B.V. All rights reserved.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 바이오나노학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17602)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.