Multiplexed detection of biomolecules using a wax printed paper-disc centrifugal optical device
- Authors
- Kim, SeJin; Kim, Dami; Kim, Sanghyo
- Issue Date
- Jan-2020
- Publisher
- ELSEVIER SCIENCE SA
- Keywords
- Colorimetric; Centrifugal; Glucose; Optical; Paper microfluidic; Wax printing
- Citation
- SENSORS AND ACTUATORS B-CHEMICAL, v.303
- Journal Title
- SENSORS AND ACTUATORS B-CHEMICAL
- Volume
- 303
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17630
- DOI
- 10.1016/j.snb.2019.127195
- ISSN
- 0925-4005
- Abstract
- In this study, we developed a wax printed paper-disc centrifugal optical device platform and simultaneously detected glucose and lactate through real-time absorbance measurements. The disposable paper module was manufactured using a simple wax printing method and was incorporated into reusable discs for colorimetric monitoring. Moreover, the paper-disc's self-calibration capability minimizes the effects of ambient conditions (ambient light, temperature, humidity and measurement time, etc.), enabling quantification with high accuracy and precision. The photodiode (PD) and light-emitting diode (LED) of the developed optical devices were able to monitor the color conversion in multiple reaction zones on a rotating paper-disc in real-time, regardless of ambient light conditions. Quantitative results were available in less than 20 s and did not require any complex image analysis. The detection limits of glucose and lactate were 27.2 mu M and 29.6 mu M, respectively, and simultaneous detection of glucose and lactate was possible even with a small serum volume (1.0 mu L). Thus, the developed wax printed paper-disc centrifugal optical device platform has promising prospects for clinical multianalyte point-of-care testing due to easy manufacturing, cost-effectiveness, user-friendliness, and high sensitivity.
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Collections - 바이오나노대학 > 바이오나노학과 > 1. Journal Articles
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