Ursonic acid exerts inhibitory effects on matrix metalloproteinases via ERK signaling pathway
- Authors
- Son J.; Lee S.Y.
- Issue Date
- Jan-2020
- Publisher
- Elsevier Ireland Ltd
- Keywords
- ERK; Keratinocyte; MAPK; Matrix metalloproteinase; Non-small cell lung cancer; Ursonic acid
- Citation
- Chemico-Biological Interactions, v.315
- Journal Title
- Chemico-Biological Interactions
- Volume
- 315
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17759
- DOI
- 10.1016/j.cbi.2019.108910
- ISSN
- 0009-2797
- Abstract
- Ursonic acid is a pentacyclic triterpenoid compound that can be extracted from Ziziphus jujuba Mill., a traditional medicine. Matrix metalloproteinases (MMPs) are involved in cancer metastasis and skin aging. Regulation of various MMPs is closely associated with mitogen-activated protein kinases (MAPKs), including ERK, p38, and JNK MAPKs. In this study, we investigated the possibility of ursonic acid as an anti-cancer/anti-skin aging agent targeting MMPs. Cytotoxic effects of ursonic acid were analyzed by cell counting kit-8 (CCK-8) assay. Invasive abilities of ursonic acid-treated A549 and H1299 non-small cell lung cancer (NSCLC) cells were tested with Boyden chamber assay. Effects of ursonic acid on MMPs were analyzed by zymography assays and quantitative real time polymerase chain reaction (qRT-PCR). We also conducted flow cytometry and western blot analysis to elucidate the mechanisms of MMP regulation by ursonic acid. Our results revealed that ursonic acid inhibited transcriptional expression of gelatinases (MMP-2 and MMP-9) via inhibition of ERK and CREB signaling pathways in NSCLC cells. Moreover, ursonic acid reduced mRNA levels of collagenase (MMP-1) via suppression of ERK and c-Fos signaling pathways in HaCaT keratinocytes. These results suggest that ursonic acid could be a potential candidate for development of an effective novel anti-cancer and anti-wrinkle agent. © 2019 Elsevier B.V.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - 바이오나노대학 > 생명과학과 > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17759)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.