Exogenous 8-hydroxydeoxyguanosine ameliorates liver fibrosis through the inhibition of Rac1-NADPH oxidase signaling
- Authors
- Shin, Seung Kak; Kim, Kyung-Ok; Kim, Se-Hee; Kwon, Oh Sang; Choi, Cheol Soo; Jeong, Sung Hwan; Kim, Yun Soo; Kim, Ju Hyun; Chung, Myung-Hee
- Issue Date
- Jun-2020
- Publisher
- WILEY
- Keywords
- 8-hydroxydeoxyguanosine; Liver fibrosis; NADPH oxidase; Rac1
- Citation
- JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
- Journal Title
- JOURNAL OF GASTROENTEROLOGY AND HEPATOLOGY
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/17827
- DOI
- 10.1111/jgh.14979
- ISSN
- 0815-9319
- Abstract
- Background and Aim Exogenous 8-hydroxydeoxyguanosine (8-OHdG) was suggested as an inhibitor of Rac1 and NADPH oxidase (NOX). The aim of this study was to evaluate the effects of the exogenous 8-OHdG on hepatic fibrogenesis in vitro and in vivo model of liver fibrosis. Methods Adult Sprague-Dawley rats were allocated to sham-operated rats (n = 7), rats that underwent bile duct ligation (BDL) (n = 6), and BDL rats treated with 8-OHdG (60 mg/kg/day by gavage, n = 6). All rats were sacrificed on day 21. Double immunofluorescence staining between either NOX1 or NOX2 and alpha-smooth muscle actin (SMA) in liver was performed. Hepatic fibrotic contents were assessed by hydroxyproline assay and quantified by Sirius red staining. In vitro, hepatic stellate cell (HSC) line LX-2 and HHSteC cells were stimulated by angiotensin II (10 mu M). The reactive oxygen species (ROS) production was measured by confocal microscopy. The expressions of NOX1, NOX2, alpha-SMA, transforming growth factor (TGF)-beta 1, and collagen I alpha were analyzed by quantitative real-time polymerase chain reaction or immunoblotting. Results The 8-OHdG treatment in BDL rats reduced the NOX1 and NOX2 protein expression, which overlapped with alpha-SMA compared with BDL rats. The 8-OHdG treatment in BDL rats significantly decreased the mRNA expression of NOX1, NOX2, alpha-SMA, TGF-beta 1, and collagen I alpha, and fibrotic contents. Increases of ROS production, Rac1 activation, NOX1, NOX2, and fibronectin expression induced by angiotensin II in HSCs were attenuated by 8-OHdG. Conclusions Rac1 activation and NOX-derived ROS are implicated to liver fibrosis. The 8-OHdG ameliorates liver fibrosis through the inhibition of Rac1 activation and NOX-derived ROS.
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