Tracking of Intentionally Inoculated Lactic Acid Bacteria Strains in Yogurt and Probiotic Powder
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Sharma, Anshul | - |
dc.contributor.author | Kaur, Jasmine | - |
dc.contributor.author | Lee, Sulhee | - |
dc.contributor.author | Park, Young-Seo | - |
dc.date.available | 2020-04-06T06:40:22Z | - |
dc.date.created | 2020-04-02 | - |
dc.date.issued | 2020-01 | - |
dc.identifier.issn | 2076-2607 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/26184 | - |
dc.description.abstract | The present work aimed at tracking intentionally inoculated lactic acid bacteria (LAB) strains in yogurt and probiotic powder. Leuconostoc (Leu.) mesenteroides (11251), Lactobacillus (L.) brevis (B151), and Lactobacillus plantarum (LB41(K)) strains were tracked in yogurt, and L. plantarum (LB41(P)) was tracked in a commercial probiotic powder. The yogurt was intentionally inoculated with the selected bacterial strains. Two types of yogurt with known and unknown bacterial pools were utilized. The standard 16S rRNA gene sequencing was used to evaluate the initial screening. The molecular typing tools, random amplified polymorphic DNA (RAPD), repetitive element palindromic PCR (rep-PCR), and comparative gene sequence analysis of selected housekeeping loci were used to track the inoculated dubious strains. Out of 30 random selections for each inoculation, the developed method identified seven (11251), nine (B151), and five (LB41(K)) colonies in the yogurt. The validation was performed by identifying 7 colonies (LB41(P)) out of 30 in the probiotic powder. The DNA banding profiles and the gene sequence alignments led to the identification of the correct inoculated strains. Overall, the study summarizes the use of molecular tools to identify the deliberately inoculated LAB strains. In conclusion, the proposed polyphasic approach effectively tracked the intentionally inoculated strains: Leu. mesenteroides, L. brevis, and L. plantarum (LB41(K)) in yogurt and L. plantarum (LB41(P)) in probiotic powder. The study demonstrates how to track industrially relevant misused LAB strains in marketable food products. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | MICROORGANISMS | - |
dc.subject | LACTOBACILLUS-PLANTARUM STRAINS | - |
dc.subject | LEUCONOSTOC-MESENTEROIDES | - |
dc.subject | MASS-SPECTROMETRY | - |
dc.subject | FOOD-PRODUCTS | - |
dc.subject | IDENTIFICATION | - |
dc.subject | DIVERSITY | - |
dc.title | Tracking of Intentionally Inoculated Lactic Acid Bacteria Strains in Yogurt and Probiotic Powder | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000514650700009 | - |
dc.identifier.doi | 10.3390/microorganisms8010005 | - |
dc.identifier.bibliographicCitation | MICROORGANISMS, v.8, no.1 | - |
dc.identifier.scopusid | 2-s2.0-85077199131 | - |
dc.citation.title | MICROORGANISMS | - |
dc.citation.volume | 8 | - |
dc.citation.number | 1 | - |
dc.contributor.affiliatedAuthor | Sharma, Anshul | - |
dc.contributor.affiliatedAuthor | Kaur, Jasmine | - |
dc.contributor.affiliatedAuthor | Lee, Sulhee | - |
dc.contributor.affiliatedAuthor | Park, Young-Seo | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | lactic acid bacteria | - |
dc.subject.keywordAuthor | Leuconostoc mesenteroides | - |
dc.subject.keywordAuthor | Lactobacillus brevis | - |
dc.subject.keywordAuthor | Lactobacillus plantarum | - |
dc.subject.keywordAuthor | tracking | - |
dc.subject.keywordAuthor | RAPD-PCR | - |
dc.subject.keywordAuthor | rep-PCR | - |
dc.subject.keywordAuthor | housekeeping genes | - |
dc.subject.keywordPlus | LACTOBACILLUS-PLANTARUM STRAINS | - |
dc.subject.keywordPlus | LEUCONOSTOC-MESENTEROIDES | - |
dc.subject.keywordPlus | MASS-SPECTROMETRY | - |
dc.subject.keywordPlus | FOOD-PRODUCTS | - |
dc.subject.keywordPlus | IDENTIFICATION | - |
dc.subject.keywordPlus | DIVERSITY | - |
dc.relation.journalResearchArea | Microbiology | - |
dc.relation.journalWebOfScienceCategory | Microbiology | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.
1342, Seongnam-daero, Sujeong-gu, Seongnam-si, Gyeonggi-do, Republic of Korea(13120)031-750-5114
COPYRIGHT 2020 Gachon University All Rights Reserved.
Certain data included herein are derived from the © Web of Science of Clarivate Analytics. All rights reserved.
You may not copy or re-distribute this material in whole or in part without the prior written consent of Clarivate Analytics.