Identification of Missing Proteins in Human Olfactory Epithelial Tissue by Liquid Chromatography-Tandem Mass Spectrometry
- Authors
- Hwang, Heeyoun; Jeong, Ji Eun; Lee, Hyun Kyoung; Yun, Ki Na; An, Hyun Joo; Lee, Bonghee; Paik, Young-Ki; Jeong, Tae Seok; Yee, Gi Taek; Kim, Jin Young; Yoo, Jong Shin
- Issue Date
- Dec-2018
- Publisher
- AMER CHEMICAL SOC
- Citation
- JOURNAL OF PROTEOME RESEARCH, v.17, no.12, pp.4320 - 4324
- Journal Title
- JOURNAL OF PROTEOME RESEARCH
- Volume
- 17
- Number
- 12
- Start Page
- 4320
- End Page
- 4324
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3004
- DOI
- 10.1021/acs.jproteome.8b00408
- ISSN
- 1535-3893
- Abstract
- We performed proteomic analyses of human olfactory epithelial tissue to identify missing proteins using liquid chromatography-tandem mass spectrometry. Using a next-generation proteomic pipeline with a < 1.0% false discovery rate at the peptide and protein levels, we identified 3731 proteins, among which five were missing proteins (POC7M7, P46721, P59826, Q658L1, and Q8N434). We validated the identified missing proteins using the corresponding synthetic peptides. No olfactory receptor (OR) proteins were detected in olfactory tissue, suggesting that detection of ORs would be very difficult. We also identified 49 and 50 alternative splicing variants mapped at the neXtProt and GENCODE databases, respectively, and 2000 additional single amino acid variants. This data set is available at the ProteomeXchange consortium via PRIDE repository (PXD010025).
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Collections - 의과대학 > 의학과 > 1. Journal Articles
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