Evaluation of in vitro anti-oxidant and anti-inflammatory activities of Korean and Chinese Lonicera caerulea
- Authors
- Lee, You-Suk; Cho, Il Je; Kim, Joo Wan; Lee, Sun-Kyoung; Ku, Sae Kwang; Lee, Hae-Jeung
- Issue Date
- Dec-2018
- Publisher
- KOREAN NUTRITION SOC
- Keywords
- Honeysuckle berry; hepatoprotective effect
- Citation
- NUTRITION RESEARCH AND PRACTICE, v.12, no.6, pp.486 - 493
- Journal Title
- NUTRITION RESEARCH AND PRACTICE
- Volume
- 12
- Number
- 6
- Start Page
- 486
- End Page
- 493
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3052
- DOI
- 10.4162/nrp.2018.12.6.486
- ISSN
- 1976-1457
- Abstract
- BACKGROUND/OBJECTIVE: The honeysuckle berry (HB) contains ascorbic acid and phenolic components, especially anthocyanins, flavonoids, and low-molecular-weight phenolic acids. In order to examine the potential of HB as a hepatoprotective medicinal food, we evaluated the in vitro anti-oxidant and anti-inflammatory activities of Korean HB (HBK) and Chinese HB (HBC). MATERIALS/METHODS: Antioxidant and anti-inflammatory effects of the extracts were examined in HepG2 and RAW 264.7 cells, respectively. The anti-oxidant capacity was determined by DPPH, SOD, CAT, and ARE luciferase activities. The production of nitric oxide (NO) as an inflammatory marker was also evaluated. The Nrf2-mediated mRNA levels of heme oxygenase-1 (HO-1), NAD(P)H dehydrogenase [quinone] 1 (Nqo1), and glutamate-cysteine ligase catalytic subunit (Gclc) were measured. The concentrations of HB extracts used were 3, 10, 30, 100, and 300 mu g/mL. RESULTS: The radical scavenging activity of all HB extracts increased in a concentration-dependent manner (P < 0.01 or P < 0.05). SOD (P < 0.05) and CAT (P < 0.01) activities were increased by treatment with 300 mu g/mL of each HB extract, when compared to those in the control. NO production was observed in cells pretreated with 100 or 300 mu g/mL of HBC and HBK (P < 0.01). Treatment with 300 mu g/mL of HBC significantly increased Nqo1 (P < 0.01) and Gclc (P < 0.05) mRNA levels compared to those in the control. Treatment with 300 mu g/mL of HBK (P < 0.05) and HBC (P < 0.01) also significantly increased the HO-1 mRNA level compared to that in the control. CONCLUSIONS: Thus, the Korean and Chinese HBs were found to possess favorable in vitro anti-oxidant and anti-inflammatory activities. Nrf2 and its related anti-oxidant genes were associated with both anti-oxidant and anti-inflammatory activities in HB-treated cells. Further studies are needed to confirm these in vivo effects.
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