Fabrication of an integrated polystyrene microdevice for pre-concentration and amplification of Escherichia coli O157:H7 from raw milk
DC Field | Value | Language |
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dc.contributor.author | Trinh, Kieu The Loan | - |
dc.contributor.author | Nguyen Minh Chon | - |
dc.contributor.author | Lee, Nae Yoon | - |
dc.date.available | 2020-02-27T08:42:00Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2018-11-14 | - |
dc.identifier.issn | 1759-9660 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3084 | - |
dc.description.abstract | In this study, we fabricated an integrated polystyrene (PS) microdevice for adhering Escherichia coli O157:H7 by exploiting the pristine PS surface as a pre-concentration step and subsequent amplification using a continuous flow polymerase chain reaction (CF-PCR) for rapid on-site detection. The PS surface, which contained styrene groups, could adhere to bacteria via electrostatic and van der Waals interactions. Subsequently, integration of pre-concentration and amplification using CF-PCR on the PS microdevice was demonstrated for rapid bacterial detection. The introduced method can be an easy-to-use platform for the rapid and simple detection of foodborne pathogens in real samples. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | ROYAL SOC CHEMISTRY | - |
dc.relation.isPartOf | ANALYTICAL METHODS | - |
dc.subject | FOODBORNE PATHOGENS | - |
dc.subject | RAPID DETECTION | - |
dc.subject | PCR | - |
dc.subject | BACTERIA | - |
dc.subject | ADHESION | - |
dc.subject | SURFACES | - |
dc.subject | PERFORMANCE | - |
dc.subject | MODEL | - |
dc.title | Fabrication of an integrated polystyrene microdevice for pre-concentration and amplification of Escherichia coli O157:H7 from raw milk | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000448948300002 | - |
dc.identifier.doi | 10.1039/c8ay01707g | - |
dc.identifier.bibliographicCitation | ANALYTICAL METHODS, v.10, no.42 | - |
dc.identifier.scopusid | 2-s2.0-85056126678 | - |
dc.citation.title | ANALYTICAL METHODS | - |
dc.citation.volume | 10 | - |
dc.citation.number | 42 | - |
dc.contributor.affiliatedAuthor | Trinh, Kieu The Loan | - |
dc.contributor.affiliatedAuthor | Lee, Nae Yoon | - |
dc.type.docType | Article | - |
dc.subject.keywordPlus | FOODBORNE PATHOGENS | - |
dc.subject.keywordPlus | RAPID DETECTION | - |
dc.subject.keywordPlus | PCR | - |
dc.subject.keywordPlus | BACTERIA | - |
dc.subject.keywordPlus | ADHESION | - |
dc.subject.keywordPlus | SURFACES | - |
dc.subject.keywordPlus | PERFORMANCE | - |
dc.subject.keywordPlus | MODEL | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalResearchArea | Food Science & Technology | - |
dc.relation.journalResearchArea | Spectroscopy | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Food Science & Technology | - |
dc.relation.journalWebOfScienceCategory | Spectroscopy | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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