A Label-Free Detection of NdeI Endonuclease Activity by Using DNA-Templated Silver Nanoclusters
- Authors
- Lee, Chunghyun; Gang, Jongback
- Issue Date
- Sep-2018
- Publisher
- AMER SCIENTIFIC PUBLISHERS
- Keywords
- Fluorescence Assay; DNA-AgNCs; NdeI Endonuclease Assay
- Citation
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY, v.18, no.9, pp.6339 - 6342
- Journal Title
- JOURNAL OF NANOSCIENCE AND NANOTECHNOLOGY
- Volume
- 18
- Number
- 9
- Start Page
- 6339
- End Page
- 6342
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3354
- DOI
- 10.1166/jnn.2018.15657
- ISSN
- 1533-4880
- Abstract
- Restriction endonucleases play an important role in genetic recombination, molecular cloning, clinical diagnosis, and pharmacological application in drug studies. In the present study, DNA-templated Ag+ nanoclusters (DNA-AgNCs) were used to detect NdeI endonuclease activity due to their facile synthesis, outstanding optical properties, and good biocompatibility. This study monitored fluorescence intensity of DNA-AgNCs to detect NdeI endonuclease activity and its inhibition in the presence of 5-fluorouracil under optimal conditions.
- Files in This Item
- There are no files associated with this item.
- Appears in
Collections - ETC > 1. Journal Articles
![qrcode](https://api.qrserver.com/v1/create-qr-code/?size=55x55&data=https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3354)
Items in ScholarWorks are protected by copyright, with all rights reserved, unless otherwise indicated.