Optimization of Oligosaccharide Production from Leuconostoc lactis Using a Response Surface Methodology and the Immunostimulating Effects of These Oligosaccharides on Macrophage Cells
DC Field | Value | Language |
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dc.contributor.author | Lee, Sulhee | - |
dc.contributor.author | Park, Gwi-Gun | - |
dc.contributor.author | Jang, Jae-Kweon | - |
dc.contributor.author | Park, Young-Seo | - |
dc.date.available | 2020-02-27T09:42:21Z | - |
dc.date.created | 2020-02-06 | - |
dc.date.issued | 2018-09 | - |
dc.identifier.issn | 1420-3049 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3455 | - |
dc.description.abstract | Production of oligosaccharides from Leuconostoc lactis CCK940 was optimized using a response surface methodology with a central composite design. Culture temperature and the concentrations of sucrose and maltose were used as the main factors. The predicted optimum conditions for the production of oligosaccharides were a culture temperature of 30 degrees C, a sucrose concentration of 9.6% (w/v), and a maltose concentration of 7.4% (w/v). Using these optimal conditions, Leuconostoc lactis CCK940 was cultured using a fermenter to produce oligosaccharides, and the resulting oligosaccharides with a degree of polymerization greater than 4 were purified by Bio-gel P2 gel permeation column chromatography and then lyophilized. When macrophages were treated with the purified oligosaccharides at concentrations of 0.1-10 mg/mL, no cytotoxicity towards the macrophages was observed. However, nitric oxide production levels were similar to those following treatment with 1 mu g/mL lipopolysaccharide. The mRNA expression levels of tumor necrosis factor-alpha, interleukin-1 beta, interleukin-6, and inducible nitric oxide synthase were all also increased in a dose-dependent manner following treatment with the oligosaccharides. These data suggest that oligosaccharides produced by Leuconostoc lactis CCK940 could be used as an immune enhancer of macrophages. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | MDPI | - |
dc.relation.isPartOf | MOLECULES | - |
dc.subject | NITRIC-OXIDE | - |
dc.subject | GLUCANSUCRASE | - |
dc.subject | FRACTION | - |
dc.subject | ACCEPTOR | - |
dc.title | Optimization of Oligosaccharide Production from Leuconostoc lactis Using a Response Surface Methodology and the Immunostimulating Effects of These Oligosaccharides on Macrophage Cells | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000447365100031 | - |
dc.identifier.doi | 10.3390/molecules23092118 | - |
dc.identifier.bibliographicCitation | MOLECULES, v.23, no.9 | - |
dc.identifier.scopusid | 2-s2.0-85052800530 | - |
dc.citation.title | MOLECULES | - |
dc.citation.volume | 23 | - |
dc.citation.number | 9 | - |
dc.contributor.affiliatedAuthor | Lee, Sulhee | - |
dc.contributor.affiliatedAuthor | Park, Gwi-Gun | - |
dc.contributor.affiliatedAuthor | Park, Young-Seo | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | response surface methodology | - |
dc.subject.keywordAuthor | central composite design | - |
dc.subject.keywordAuthor | oligosaccharide | - |
dc.subject.keywordAuthor | optimization | - |
dc.subject.keywordAuthor | immunostimulation | - |
dc.subject.keywordAuthor | RAW264.7 | - |
dc.subject.keywordAuthor | lactic acid bacteria | - |
dc.subject.keywordPlus | NITRIC-OXIDE | - |
dc.subject.keywordPlus | GLUCANSUCRASE | - |
dc.subject.keywordPlus | FRACTION | - |
dc.subject.keywordPlus | ACCEPTOR | - |
dc.relation.journalResearchArea | Biochemistry & Molecular Biology | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Biochemistry & Molecular Biology | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Multidisciplinary | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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