Fluorometric 'switch-on' detection of heparin based on a system composed of rhodamine-labeled chitosan oligosaccharide lactate, and graphene oxide
DC Field | Value | Language |
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dc.contributor.author | Zhong, Linlin | - |
dc.contributor.author | Yun, Kyusik | - |
dc.date.available | 2020-02-27T10:41:51Z | - |
dc.date.created | 2020-02-07 | - |
dc.date.issued | 2018-06 | - |
dc.identifier.issn | 2050-6120 | - |
dc.identifier.uri | https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3686 | - |
dc.description.abstract | A novel fluorescence 'Switch on' for the detection of heparin based on the RhB-COL/GO system was achieved. A strong fluorescence dye, Rhodamine B, was modified by chitosan oligosaccharide lactate (COL), which plays a major role in the formation of a positively charged RhB-COL complex. RhB-COL was soluble and stable in solution, which was characterized by using Fourier transform infrared spectroscopy and x-ray photoelectron spectroscopy. GO sheets quenched the fluorescence intensity of RhB-COL due to electron transfer from RhB to the GO surface. The decrease in fluorescence intensity of RhB-COL with increasing GO concentration was recorded using a Cary Eclipse fluorescence spectrophotometer. On the other hand, the addition of heparin replaced GO to bind with the RhB-COL surface via an electrostatic and noncovalent bond due to the abundant negative charge, which resulted in recovery of the fluorescence intensity. This RhB-COL/GO system possessed high selectivity and good sensitivity for the detection of heparin compared to other biomolecules, such as glycine, D-glucose, hyaluronic acid, L-glutamic acid, and ascorbic acid. The linear response toward heparin was measured over the range, 0-1.8 U . ml(-1), with a low detection limit of 0.04 U . ml(-1). The satisfactory sensing performance of RhB-COL/GO for heparin supports new 'switch-on' sensor applications in heparin-related biomedical detection. | - |
dc.language | 영어 | - |
dc.language.iso | en | - |
dc.publisher | IOP PUBLISHING LTD | - |
dc.relation.isPartOf | METHODS AND APPLICATIONS IN FLUORESCENCE | - |
dc.subject | OVERSULFATED CHONDROITIN SULFATE | - |
dc.subject | SELECTIVE DETECTION | - |
dc.subject | QUANTUM DOTS | - |
dc.subject | RATIOMETRIC DETECTION | - |
dc.subject | SENSITIVE DETECTION | - |
dc.subject | FLUORESCENCE PROBE | - |
dc.subject | AQUEOUS-SOLUTION | - |
dc.subject | VISUAL DETECTION | - |
dc.subject | SENSOR | - |
dc.subject | CONTAMINANT | - |
dc.title | Fluorometric 'switch-on' detection of heparin based on a system composed of rhodamine-labeled chitosan oligosaccharide lactate, and graphene oxide | - |
dc.type | Article | - |
dc.type.rims | ART | - |
dc.description.journalClass | 1 | - |
dc.identifier.wosid | 000433499800001 | - |
dc.identifier.doi | 10.1088/2050-6120/aac51c | - |
dc.identifier.bibliographicCitation | METHODS AND APPLICATIONS IN FLUORESCENCE, v.6, no.3 | - |
dc.identifier.scopusid | 2-s2.0-85051412875 | - |
dc.citation.title | METHODS AND APPLICATIONS IN FLUORESCENCE | - |
dc.citation.volume | 6 | - |
dc.citation.number | 3 | - |
dc.contributor.affiliatedAuthor | Zhong, Linlin | - |
dc.contributor.affiliatedAuthor | Yun, Kyusik | - |
dc.type.docType | Article | - |
dc.subject.keywordAuthor | Rhodamine B | - |
dc.subject.keywordAuthor | RhB-COL/GO | - |
dc.subject.keywordAuthor | heparin | - |
dc.subject.keywordAuthor | fluorescence quenching | - |
dc.subject.keywordAuthor | switch on | - |
dc.subject.keywordPlus | OVERSULFATED CHONDROITIN SULFATE | - |
dc.subject.keywordPlus | SELECTIVE DETECTION | - |
dc.subject.keywordPlus | QUANTUM DOTS | - |
dc.subject.keywordPlus | RATIOMETRIC DETECTION | - |
dc.subject.keywordPlus | SENSITIVE DETECTION | - |
dc.subject.keywordPlus | FLUORESCENCE PROBE | - |
dc.subject.keywordPlus | AQUEOUS-SOLUTION | - |
dc.subject.keywordPlus | VISUAL DETECTION | - |
dc.subject.keywordPlus | SENSOR | - |
dc.subject.keywordPlus | CONTAMINANT | - |
dc.relation.journalResearchArea | Chemistry | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Analytical | - |
dc.relation.journalWebOfScienceCategory | Chemistry, Physical | - |
dc.description.journalRegisteredClass | scie | - |
dc.description.journalRegisteredClass | scopus | - |
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