Fluorometric 'switch-on' detection of heparin based on a system composed of rhodamine-labeled chitosan oligosaccharide lactate, and graphene oxide
- Authors
- Zhong, Linlin; Yun, Kyusik
- Issue Date
- Jun-2018
- Publisher
- IOP PUBLISHING LTD
- Keywords
- Rhodamine B; RhB-COL/GO; heparin; fluorescence quenching; switch on
- Citation
- METHODS AND APPLICATIONS IN FLUORESCENCE, v.6, no.3
- Journal Title
- METHODS AND APPLICATIONS IN FLUORESCENCE
- Volume
- 6
- Number
- 3
- URI
- https://scholarworks.bwise.kr/gachon/handle/2020.sw.gachon/3686
- DOI
- 10.1088/2050-6120/aac51c
- ISSN
- 2050-6120
- Abstract
- A novel fluorescence 'Switch on' for the detection of heparin based on the RhB-COL/GO system was achieved. A strong fluorescence dye, Rhodamine B, was modified by chitosan oligosaccharide lactate (COL), which plays a major role in the formation of a positively charged RhB-COL complex. RhB-COL was soluble and stable in solution, which was characterized by using Fourier transform infrared spectroscopy and x-ray photoelectron spectroscopy. GO sheets quenched the fluorescence intensity of RhB-COL due to electron transfer from RhB to the GO surface. The decrease in fluorescence intensity of RhB-COL with increasing GO concentration was recorded using a Cary Eclipse fluorescence spectrophotometer. On the other hand, the addition of heparin replaced GO to bind with the RhB-COL surface via an electrostatic and noncovalent bond due to the abundant negative charge, which resulted in recovery of the fluorescence intensity. This RhB-COL/GO system possessed high selectivity and good sensitivity for the detection of heparin compared to other biomolecules, such as glycine, D-glucose, hyaluronic acid, L-glutamic acid, and ascorbic acid. The linear response toward heparin was measured over the range, 0-1.8 U . ml(-1), with a low detection limit of 0.04 U . ml(-1). The satisfactory sensing performance of RhB-COL/GO for heparin supports new 'switch-on' sensor applications in heparin-related biomedical detection.
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