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The detection of a mismatched DNA by using hairpin DNA-templated silver nanoclusters
- Authors
- Kim, Seyeon; Gang, Jongback
- Issue Date
- May-2018
- Publisher
- ACADEMIC PRESS INC ELSEVIER SCIENCE
- Keywords
- DNA detection; Biosensor; DNA-Templated silver nanoclusters (DNA-AgNCs); Hairpin DNA
- Citation
- ANALYTICAL BIOCHEMISTRY, v.549, pp.171 - 173
- Journal Title
- ANALYTICAL BIOCHEMISTRY
- Volume
- 549
- Start Page
- 171
- End Page
- 173
- ISSN
- 0003-2697
- Abstract
- Fluorescence assays have been developed to detect biomolecules using DNA-templated silver nanoclusters (DNA-AgNCs) utilizing their unique physical and optical properties. This study was designed to detect single-mismatched DNA by the hybridization of target DNA to template DNA either before or after DNA-AgNCs synthesis. The results showed that the detection specificity of a single-mismatched DNA was clearly enhanced when the target DNA (cDNA) was hybridized to template DNA prior to DNA-AgNCs synthesis compared with cDNA hybridization subsequent to DNA-AgNCs synthesis.
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Related Researcher
- Gang, Jong Back
- BioNano Technology (Department of Chemistry)